ArticleScientific reports2023
High-resolution microbiome analysis reveals exclusionary Klebsiella species competition in preterm infants at risk for necrotizing enterocolitis.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 16 citations in OpenAlex.
- Gut Microbiome in Neonatal Necrotizing Enterocolitis - A Comprehensive Review of Evidence.Indian journal of pediatrics · 2026Review
- Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis.Microbiology spectrum · 2026Article
- Clostridia from preterm infants metabolize human milk oligosaccharides to suppress pathobionts and modulate intestinal function in organoids.Nature microbiology · 2026Article
- Tracing the early bacterial settlers in preterm and very-low birth-weight infants: first report of microbiota dynamics in South American neonates.Infection and immunity · 2026Article
- Transcriptional stratification of necrotizing enterocolitis identifies distinct disease subtypes.Scientific reports · 2026Article
- Chemokine-receptor-dependent inflammatory responses contribute to two-hit-induced experimental necrotizing enterocolitis and represent a potential therapeutic target.Frontiers in immunology · 2026Article
- Refining microbiome resolution: KoSCAPEGut microbes · 2025Article
- Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis.bioRxiv : the preprint server for biology · 2025Article
- Source-tracking Klebsiella outbreaks in premature infants using a novel amplicon fingerprinting method.Antimicrobial resistance and infection control · 2025Article
- Profiling the response of individual gut microbes to free fatty acids (FFAs) found in human milk.Journal of functional foods · 2025Article
- Microbiota regulates neonatal disease tolerance to virus-evoked necrotizing enterocolitis by shaping the STAT1-NLRC5 axis in the intestinal epithelium.Cell host & microbe · 2024Article
- Core genome multilocus sequence typing (cgMLST) applicable to the monophyleticJournal of clinical microbiology · 2024Article
- Antimicrobial susceptibility profile ofVeterinary world · 2024Article
- Unexpected decrease in necrotizing enterocolitis morbidity during the COVID-19 pandemic-A single-centre retrospective study.Frontiers in pediatrics · 2024Article
- Characterization of a Highly VirulentInfection and drug resistance · 2024Article
- Microbiota-derived small molecule genotoxins: host interactions and ecological impact in the gut ecosystem.Gut microbesReview
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal colonization with Klebsiella has been linked to necrotizing enterocolitis (NEC), but methods of analysis usually failed to discriminate Klebsiella species or strains. A novel ~ 2500-base amplicon (StrainID) that spans the 16S and 23S rRNA genes was used to generate amplicon sequence variant (ASV) fingerprints for Klebsiella oxytoca and Klebsiella pneumoniae species complexes (KoSC and KpSC, respectively) and co-occurring fecal bacterial strains from 10 preterm infants with NEC and 20 matched controls. Complementary approaches were used to identify cytotoxin-producing isolates of KoSC. Klebsiella species colonized most preterm infants, were more prevalent in NEC subjects versus controls, and replaced Escherichia in NEC subjects. Single KoSC or KpSC ASV fingerprinted strains dominated the gut microbiota, suggesting exclusionary Klebsiella competition for luminal resources. Enterococcus faecalis was co-dominant with KoSC but present infrequently with KpSC. Cytotoxin-producing KoSC members were identified in most NEC subjects and were less frequent in controls. Few Klebsiella strains were shared between subjects. We conclude that inter-species Klebsiella competition, within an environment of KoSC and E. faecalis cooperation, appears to be an important factor for the development of NEC. Preterm infants seem to acquire Klebsiella primarily through routes other than patient-to-patient transmission.
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