Evidence map›Paper›PMID 37193330›Full record

Review3 Biotech2023

A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and environmental sustainability.

Fernanda Leonhardt, Adriano Gennari, Graziela Barbosa Paludo, Caroline Schmitz, Filipe Xerxeneski da Silveira, Débora Cristina Daenecke Albuquerque Moura, Gaby Renard, Giandra Volpato, Claucia Fernanda Volken de Souza

Open access · greenAbstract readReview
In one paragraph

Review in 3 Biotech, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
5.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Semiartificial COChemical reviews · 2026
    Review
  2. Article
  3. Novel NTA-NiPolymers · 2026
    Article
  4. Scaling Cultured Meat: Challenges and Solutions for Affordable Mass Production.Comprehensive reviews in food science and food safety · 2025
    Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Fernanda LeonhardtFood Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.
Adriano GennariFood Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.
Graziela Barbosa PaludoFood Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.
Caroline SchmitzFood Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.
Filipe Xerxeneski da SilveiraFederal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil.
Débora Cristina Daenecke Albuquerque MouraFederal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil.
Gaby RenardQuatro G Pesquisa & Desenvolvimento Ltda, Porto Alegre, RS Brazil.
Giandra VolpatoFederal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil.
Claucia Fernanda Volken de SouzaFood Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.ORCID 0000-0001-7708-3762
Univates · BRUniversidade Federal do Rio Grande do Sul · BRFaculdade de Desenvolvimento do Rio Grande do Sul · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach used to structure this systematic review was The Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) methodology. The Scopus and Web of Science databases were used to perform the bibliographic survey by which 267 articles were selected. After the inclusion/exclusion criteria and the screening process, from 25 chosen documents, we identified 7 types of tags used in the last 10 years, carbohydrate-binding module tag (CBM), polyhistidine (His-tag), elastin-like polypeptides (ELPs), silaffin-3-derived pentalysine cluster (Sil3k tag), N-acetylmuramidase (AcmA tag), modified haloalkane dehalogenase (HaloTag

Indexed as

Cloning vectorsEnzyme immobilizationExpression hostFusion tagPlasmid labelSingle-step purification

Identifiers

PMID37193330
PMCPMC10182917
OpenAlexW4376616555

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.