Evidence map›Paper›PMID 37193170›Full record

ArticleAmerican journal of translational research2023

TSF/FHA induces osteogenic differentiation of Mc3t3 cells via Pygo2 dependent Wnt/β-catenin signaling pathway.

Wenhang Dong, Rui Li, Minglei Sun, Hongyan Mi, Meiyue Wang, Mengzhe Liu, Weiliu Qiu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
–field-weighted citation impact, top 76% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Wenhang DongDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Rui LiDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Minglei SunDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Hongyan MiDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Meiyue WangDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Mengzhe LiuDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Weiliu QiuDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, Henan, China.
Shanghai Ninth People's Hospital · CNFirst Affiliated Hospital of Zhengzhou University · CNShanghai Stomatological Hospital · CNZhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study aimed to investigate whether tussah silk fibroin (TSF)/fluoridated hydroxyapatite (FHA) can promote osteogenic differentiation of Mc3t3 cells and explore the role of Wnt/β-catenin signaling in this process.

methodsTSF/FHA was gained via freeze drying technique and cyclic phosphate immersion method. The relative expression levels of bone-related genes and proteins of Mc3t3 cells seeded on different materials were examined by RT-qPCR and Western blotting. Knockdown or overexpression of Pygo2 in Mc3t3 cells was achieved using lentiviral transfection. Cell proliferation, the expression of bone-related genes and proteins were subsequently examined. Animal experiment was also performed to observe the osteogenesis effect.

resultsDifferent ratios of fluorine of TSF/FHA accelerated the osteogenic differentiation of Mc3t3 cells and increased the Pygo2 expression. The Wnt/β-catenin signaling pathway was activated after TSF/FHA induction, accompanied by the increased expression of related genes. In SD rats with skull defect, the newly formed bone increased significantly and the Pygo2 overexpressing Mc3t3 cells promoted osteogenesis. However, Pygo2 knockdown markedly compromised the osteogenesis of Mc3t3 cells after TSF/FHA induction.

conclusionTSF/FHA facilitates osteogenic differentiation of Mc3t3 cells via upregulating Pygo2 and activating Wnt/β-catenin signaling pathway.

Indexed as

fluorapatite and hydroxyapatitefluoro-hydroxyapatiteMc3t3osteogenesisSilk fibroin

Identifiers

PMID37193170
PMCPMC10182493
OpenAlexW4376872534

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.