ArticleIEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy.
Article in IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Photon absorption remote sensing (PARS): comprehensive absorption imaging enabling label-free biomolecule characterization and mapping.Scientific reports · 2026Article
- Pixel-level annotations of context region for trustworthy diagnosis of Breast Lymph Node Metastasis from Histopathological WSI.Data in brief · 2026Article
- Bridging the Gap Between Static Histology and Dynamic Organ-on-a-Chip Models.Pathophysiology : the official journal of the International Society for Pathophysiology · 2026Review
- Rapid cancer diagnosis using deep learning-powered label-free subcellular-resolution photoacoustic histology.Science advances · 2025Article
- Translating Features to Findings: Deep Learning for Melanoma Subtype Prediction.Dermatopathology (Basel, Switzerland) · 2025Review
- Deep learning-based classification of breast cancer molecular subtypes from H&E whole-slide images.Journal of pathology informatics · 2025Article
- Large field-of-view metabolic profiling of murine brain tissue following morphine incubation using label-free multiphoton microscopy.Journal of neuroscience methods · 2024Article
- Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal.Biomedical optics express · 2024Article
- Corrections to "Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy".IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Hematoxylin and eosin (H&E) staining, the century-old technique, has been the gold standard tool for pathologists to detect anomalies in tissues and diseases such as cancer. H&E staining is a cumbersome, time-consuming process that delays and wastes precious minutes during an intraoperative diagnosis. However, even in the modern era, real-time label-free imaging techniques such as simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy have delivered several more layers of information to characterize a tissue with high precision. Still, they have yet to translate to the clinic. The slow translation rate can be attributed to the lack of direct comparisons between the old and new techniques. Our approach to solving this problem is to: 1) reduce dimensions by pre-sectioning the tissue in 500 μm slices, and 2) produce fiducial laser markings which appear in both SLAM and histological imaging. High peak-power femtosecond laser pulses enable ablation in a controlled and contained manner. We perform laser marking on a grid of points encompassing the SLAM region of interest. We optimize laser power, numerical aperture, and timing to produce axially extended marking, hence multilayered fiducial markers, with minimal damage to the surrounding tissues. We performed this co-registration over an area of 3 × 3 mm
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