ArticleACS synthetic biology2023
Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate.
Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Applications of synthetic biology in biomedicine.Molecular biomedicine · 2026Review
- Electric field-guided random-access DNA data storage.Science advances · 2026Article
- Review
- Biotechnological tools boost the functional diversity of DNA-based data storage systems.Computational and structural biotechnology journal · 2025Review
- What's in a word? Defining "gene therapy medicines".Molecular therapy. Methods & clinical development · 2024Article
- A Massively ParallelACS synthetic biology · 2024Article
- Template-dependent DNA ligation for the synthesis of modified oligonucleotides.Nature communications · 2024Article
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Controlled enzymatic synthesis of oligonucleotides.Communications chemistry · 2024Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel enzymatic methods are poised to become the dominant processes for de novo synthesis of DNA, promising functional, economic, and environmental advantages over the longstanding approach of phosphoramidite synthesis. Before this can occur, however, enzymatic synthesis methods must be parallelized to enable production of multiple DNA sequences simultaneously. As a means to this parallelization, we report a polymerase-nucleotide conjugate that is cleaved using electrochemical oxidation on a microelectrode array. The developed conjugate maintains polymerase activity toward surface-bound substrates with single-base control and detaches from the surface at mild oxidative voltages, leaving an extendable oligonucleotide behind. Our approach readies the way for enzymatic DNA synthesis on the scale necessary for DNA-intensive applications such as DNA data storage or gene synthesis.
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Registered trials
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