Evidence map›Paper›PMID 37191799›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Isolation and Identification of Plasma Extracellular Vesicles Protein Biomarkers.

Michelle V Lihon, Marco Hadisurya, Xiaofeng Wu, Anton Iliuk, W Andy Tao

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michelle V LihonDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA.
Marco HadisuryaDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA.
Xiaofeng WuDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.
Anton IliukDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA.
W Andy TaoDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA. watao@purdue.edu.

Funding

Dissecting signaling pathways and seeking EV phosphoproteins as novel biomarkers for Alzheimer's DiseaseRF1AG064250 · NIA · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2020 to 2021
$1.7M
NIA NIH HHS RF1 AG064250
6 · The paper itself

Abstract

Extracellular vesicles (EVs) have emerged as a valuable source for disease biomarkers and an alternative drug delivery system due to their ability to carry cargo and target specific cells. Proper isolation, identification, and analytical strategy are required for evaluating their potential in diagnostics and therapeutics. Here, a method is detailed to isolate plasma EVs and analyze their proteomic profiling, combining EVtrap-based high-recovery EV isolation, phase-transfer surfactant method for protein extraction, and mass spectrometry qualitative and quantitative strategies for EV proteome characterization. The pipeline provides a highly effective EV-based proteome analysis technique that can be applied for EV characterization and evaluation of EV-based diagnosis and therapy.

Indexed as

Extracellular VesiclesProteomeBiomarkersMass SpectrometryProteomicsBiomarkersProteomeAutomatic gain control (AGC) strategyData-dependent acquisition (DDA)Data-independent acquisition (DIA)ExosomesExtracellular vesicles (EVs)Extracellular vesicles total recovery and purification (EVtrap)Gas-phase fractionated (GPF) spectral libraryIsobaric labelingMass spectrometryParallel reaction monitoring (PRM)PharmacokineticsPlasmaQuantitative proteomicsSpectronaut Pulsar searchTandem mass tag labeling (TMT labeling)

Identifiers

PMID37191799

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.