Evidence map›Paper›PMID 37191791›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

High-Plex Spatial Profiling of RNA and Protein Using Digital Spatial Profiler.

Nan Wang, Xia Li, Zhiyong Ding

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nan WangMills Institute for Personalized Cancer Care, Fynn Biotechnologies Ltd, Jinan City, Shandong Province, P. R. China.
Xia LiMills Institute for Personalized Cancer Care, Fynn Biotechnologies Ltd, Jinan City, Shandong Province, P. R. China.
Zhiyong DingMills Institute for Personalized Cancer Care, Fynn Biotechnologies Ltd, Jinan City, Shandong Province, P. R. China. zhiyong.ding@fynnbio.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rapid emergence of spatial multi-omics technologies in recent years has revolutionized biomedical research. Among these, the Digital Spatial Profiler (DSP, commercialized by nanoString) has become one of the dominant technologies in spatial transcriptomics and proteomics and has assisted in deconvoluting complex biological questions. Based on our practical experience in the past 3 years with DSP, we share here a detailed hands-on protocol and key handling notes that will allow the broader community to optimize their work procedure.

Indexed as

Gene Expression ProfilingRNAFormaldehydeParaffin EmbeddingTissue FixationFormaldehydeRNACancer Transcriptome Atlas (CTA)Companion diagnostics (CDs)Digital Spatial Profiler (DSP), Whole Transcriptome Atlas (WTA)Next-generation sequencing (NGS)-based barcodeSpatial biology, Transcriptomics, Proteomics, Formalin-fixed paraffin-embedded (FFPE) specimens

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.