Evidence map›Paper›PMID 37191714›Full record

ArticleAnalytical and bioanalytical chemistry2023

Hydrogel microenvironment contributes to chemical-induced differentiation of mesenchymal stem cells: single-cell infrared microspectroscopy characterization.

Jiang Qian, Yadi Wang, Xueling Li, Junhong Lü

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 1 country.

Jiang Qian *Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.
Yadi Wang *Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250000, China.
Xueling LiShanghai University of Medicine & Health Sciences, Shanghai, 201318, China.
Junhong LüShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China. lvjunhong@jnl.ac.cn.ORCID http://orcid.org/0000-0003-2873-3137
Binzhou Medical University · CNBinzhou University · CNChinese Academy of Sciences · CNShanghai University of Medicine and Health Sciences · CN

Funding

National Natural Science Foundation of China U2230110Natural Science Foundation of Shandong Province ZR2021QC029
6 · The paper itself

Abstract

Stem cell microenvironment plays vital roles in directing cell proliferation and differentiation. Due to the tiny biochemical changes in the early stage of stem cell development, technical challenges to characterize the potential effects of environmental signals remain. In this work, we have introduced synchrotron radiation-based Fourier transform infrared microspectroscopy to evaluate the synergistic effects of physical and chemical factors on stem cell differentiation at the single-cell level. By using principal component analysis and cell-cell Euclidean distance calculation, the phenotypic heterogeneity changes during stem cell osteogenesis induced by lithium chloride or Wnt5a protein loaded in the polyvinyl alcohol (PVA) hydrogel were characterized in detail. The results demonstrated that PVA hydrogel could lead to the distinct effects between low-concentration lithium and wnt5a on human mesenchymal stem cells, suggesting a vital role of niche signals in Wnt pathway. These findings highlight the importance of microenvironment to the chemical-induced effects on stem cell differentiation and also provide a label-free, noninvasive method to sensitively identify the niche function in stem cell biology.

Indexed as

HydrogelsMesenchymal Stem CellsCell DifferentiationHumansOsteogenesisPolyvinyl AlcoholStem CellsHydrogelsPolyvinyl Alcoholpolyvinyl alcohol hydrogelFourier transform infrared microspectroscopyHydrogel microenvironmentMesenchymal stem cellsOsteogenic differentiationWnt signaling

Identifiers

PMID37191714
OpenAlexW4376642547

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.