ArticleBiomedicines2023
3D Spheroid Cultivation Alters the Extent and Progression of Osteogenic Differentiation of Mesenchymal Stem/Stromal Cells Compared to 2D Cultivation.
Article in Biomedicines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Uncovering the Potential of 3D Spheroids for Modeling the Cellular Crosstalk Within the Tumor Microenvironment in HNSCC.Biotechnology journal · 2026Review
- New Approach Methodologies (NAMs) in Alternative Methods: A Comparative Cytotoxicity Analysis of Nanostructured Hydroxyapatite in Adipose Stem Cells Spheroids.Journal of applied toxicology : JAT · 2026Article
- Self-Assembled Nanoclay Gel with Spheroid MSC-Derived Exosome Mimetics to Integrate Demineralized Bone Matrix and Noggin-Targeting miRNA for Synergistic Osteogenesis.Advanced functional materials · 2026Article
- Hydroxyapatite-Mediated Mechanical Modulation of GelMA Hydrogels Influences Osteogenic Differentiation of 3D Spheroids.Gels (Basel, Switzerland) · 2026Article
- Mitochondrial Imaging and Transcriptome Analysis of Bone Mesenchymal Stem Cells During Osteogenesis Under Different Culture Conditions.Biomolecules · 2025Article
- Real-Time Visualization of Endogenous HChemical & biomedical imaging · 2025Article
- Impact of Dexamethasone on Three-Dimensional Stem Cell Spheroids: Morphology, Viability, Osteogenic Differentiation.Medicina (Kaunas, Lithuania) · 2025Article
- Current perspectives on the dynamic culture of mesenchymal stromal/stem cell spheroids.Stem cells translational medicine · 2025Review
- Modulation of Stem Cell Survival and Engraftment: Implications for Stem Cell-Based Therapy.Theranostics · 2025Review
- Impact of Vascular Endothelial Growth Factor on the Shape, Survival, and Osteogenic Transformation of Gingiva-Derived Stem Cell Spheroids.Medicina (Kaunas, Lithuania) · 2024Article
- 3D in vitro synovial hyperplasia model on polycaprolactone-micropatterned nanofibrous microwells for screening disease-modifying anti-rheumatic drugs.Materials today. Bio · 2024Article
- Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research.International journal of molecular sciences · 2024Review
- Co-culture of STRO1 + human gingival mesenchymal stem cells and human umbilical vein endothelial cells in 3D spheroids: enhancedFrontiers in cell and developmental biology · 2024Article
- Engineering three-dimensional bone macro-tissues by guided fusion of cell spheroids.Frontiers in endocrinology · 2023Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Mesenchymal stem/stromal cells (MSC) are capable of progenitor cell fraction renewal or tissue-specific differentiation. These properties are maintained during in vitro cultivation, making them an interesting model system for testing biological and pharmacological compounds. Cell cultivation in 2D is commonly used to study cellular responses, but the 2D environment does not reflect the structural situation of most cell types. Therefore, 3D culture systems have been developed to provide a more accurate physiological environment in terms of cell-cell interactions. Since knowledge about the effects of 3D culture on specific differentiation processes is limited, we studied the effects on osteogenic differentiation and the release of factors affecting bone metabolism for up to 35 days and compared them with the effects in 2D culture. We demonstrated that the selected 3D model allowed the rapid and reliable formation of spheroids that were stable over several weeks and both accelerated and enhanced osteogenic differentiation compared with the 2D culture. Thus, our experiments provide new insights into the effects of cell arrangement of MSC in 2D and 3D. However, due to the different culture dimensions, various detection methods had to be chosen, which in principle limits the explanatory power of the comparison between 2D and 3D cultures.
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