Evidence map›Paper›PMID 37189053›Full record

ArticleBMC plant biology2023

Magnesium chelatase subunit D is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting starch accumulation in Manihot esculenta Crantz.

Xingai Yang, Jie Cai, Jingjing Xue, Xiuqin Luo, Wenli Zhu, Xinhui Xiao, Maofu Xue, Feifei An, Kaimian Li, Songbi Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Xingai Yang *Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Jie Cai *Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Jingjing XueKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Xiuqin LuoKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Wenli ZhuKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Xinhui XiaoKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Maofu XueKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China.
Feifei AnKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China. aff85110@163.com.
Kaimian LiKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China. kaimianli@sohu.com.
Songbi ChenKey Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China. songbichen@catas.cn.
Tropical Crops Genetic Resources Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMagnesium chelatase plays an important role in photosynthesis, but only a few subunits have been functionally characterized in cassava.

resultsHerein, MeChlD was successfully cloned and characterized. MeChlD encodes a magnesium chelatase subunit D, which has ATPase and vWA conservative domains. MeChlD was highly expressed in the leaves. Subcellular localization suggested that MeChlD:GFP was a chloroplast-localized protein. Furthermore, the yeast two-hybrid system and BiFC analysis indicated that MeChlD interacts with MeChlM and MePrxQ, respectively. VIGS-induce silencing of MeChlD resulted in significantly decreased chlorophyll content and reduction the expression of photosynthesis-related nuclear genes. Furthermore, the storage root numbers, fresh weight and the total starch content in cassava storage roots of VIGS-MeChlD plants was significantly reduced.

conclusionTaken together, MeChlD located at the chloroplast is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting the starch accumulation in cassava. This study expands our understanding of the biological functions of ChlD proteins.

Indexed as

ManihotStarchChlorophyllLyasesPhotosynthesisChlorophyllLyasesmagnesium chelataseStarchCassava (Manihot esculenta Crantz)Chlorophyll synthesisMeChlDPhotosynthesisStarch accumulationVIGS

Identifiers

PMID37189053
PMCPMC10186761
OpenAlexW4376641713

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.