ArticleISME communications2023
Optimizing ex vivo culture conditions to study human gut microbiome.
Article in ISME communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Design and application of synthetic human gut microbial communities.Gut microbes · 2025Review
- Article
- Inter-species and individualized biotransformation of five saponins by human being- and mouse-derived fecal microbiota.Chinese medicine · 2025Article
- Gut microbiota-based bioassay for the quality evaluation of different species of Dendrobium and their therapeutic potential in type 2 diabetes.Chinese medicine · 2025Article
- Discovery and cultivation of prokaryotic taxa in the age of metagenomics and artificial intelligence.The ISME journal · 2025Article
- Differential impacts of hemin and free iron on amoxicillin susceptibility inFrontiers in microbiology · 2025Article
- Zhi-Kang-Yin formula attenuates high-fat diet-induced metabolic disorders through modulating gut microbiota-bile acids axis in mice.Chinese medicine · 2024Article
- Making in vitro conditions more reflective of in vivo conditions for research on the teleost gastrointestinal tract.The Journal of experimental biology · 2024Review
- Cultured fecal microbial community and its impact as fecal microbiota transplantation treatment in mice gut inflammation.Applied microbiology and biotechnology · 2024Article
- Advances in the isolation, cultivation, and identification of gut microbes.Military Medical Research · 2024Review
- Core microbiome-associated proteins associated with ulcerative colitis interact with cytokines for synergistic or antagonistic effects on gut bacteria.The ISME journal · 2024Article
- A flexible high-throughput cultivation protocol to assess the response of individuals' gut microbiota to diet-, drug-, and host-related factors.ISME communications · 2024Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inter-individual variations of gut microbiome contribute to the different responses toward drug therapy among populations, developing a reliable ex vivo culture method for mixed bacteria is the urgent need for predicting personal reaction to drug therapy. Unfortunately, very few attentions have been paid to the bias that could be introduced during the culture process for mixed bacteria. Here we systemically evaluated the factors that may affect the outcomes of cultured bacteria from human feces. We demonstrated that inter-individual difference of host gut microbiome was the main factor affecting the outcomes of cultured bacteria, followed by the culture medium and time point. We further optimized a new medium termed GB based on our established multi-dimensional evaluation method, which could mimic the status of in situ host gut microbiome to the highest extent. Finally, we assessed the inter-individual metabolism by host gut microbiome from 10 donors on three frequently used clinical drugs (aspirin, levodopa and doxifluridine) based on the optimized GB medium. Our results revealed obvious variation in drug metabolism by microbiome from different donors, especially levodopa and doxifluridine. This work suggested the optimized culture medium had the potential for exploring the inter-individual impacts of host gut microbiome on drug metabolism.
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Registered trials
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