Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs.

Polina I Koroleva, Andrei A Gilep, Sergey V Kraevsky, Tatiana V Tsybruk, Victoria V Shumyantseva

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In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

3.4field-weighted citation impact, top 8% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 14 citations in OpenAlex.

Engineering Electron Transfer Pathway of Cytochrome P450s.Molecules (Basel, Switzerland) · 2024
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Polina I KorolevaInstitute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, 119121 Moscow, Russia.ORCID 0000-0003-2802-5747

Andrei A GilepInstitute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, 119121 Moscow, Russia.

Sergey V KraevskyInstitute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, 119121 Moscow, Russia.

Tatiana V TsybrukInstitute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 220141 Minsk, Belarus.

Victoria V ShumyantsevaInstitute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, 119121 Moscow, Russia.ORCID 0000-0002-1509-7218

Institute of Biomedical Chemistry · RUNational Academy of Sciences of Belarus · BYPirogov Russian National Research Medical University · RU

Funding

Russian Science Foundation 23-25-00064

6 · The paper itself

Abstract

In the present work, screen-printed electrodes (SPE) modified with a synthetic surfactant, didodecyldimethylammonium bromide (DDAB) and streptolysin O (SLO) were prepared for cytochrome P450 3A4 (CYP3A4) immobilization, direct non-catalytic and catalytic electrochemistry. The immobilized CYP3A4 demonstrated a pair of redox peaks with a formal potential of -0.325 ± 0.024 V (vs. the Ag/AgCl reference electrode). The electron transfer process showed a surface-controlled mechanism ("protein film voltammetry") with an electron transfer rate constant (k

Indexed as

Cytochrome P-450 CYP3AMembrane ProteinsBacterial ProteinsElectrodesErythromycinQuaternary Ammonium CompoundsStreptolysinsBacterial ProteinsCytochrome P-450 CYP3AdidodecyldimethylammoniumErythromycinMembrane ProteinsQuaternary Ammonium Compoundsstreptolysin OStreptolysinsbioreactorcytochrome P450 3A4electrocatalysiselectrochemical analysiselectron transfererythromycinstreptolysin O

Identifiers

PMID37185532

DOI10.3390/bios13040457

PMCPMC10136652

OpenAlexW4362611120

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.