Evidence map›Paper›PMID 37185019›Full record

ArticleACS chemical biology2023

Development, Characterization, and Structural Analysis of a Genetically Encoded Red Fluorescent Peroxynitrite Biosensor.

Yu Pang, Mian Huang, Yichong Fan, Hsien-Wei Yeh, Ying Xiong, Ho Leung Ng, Hui-Wang Ai

Abstract read
In one paragraph

Article in ACS chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yu PangCenter for Membrane and Cell Physiology, University of Virginia, Charlottesville, Virginia 22908, United States.
Mian HuangDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas 66506, United States.
Yichong FanCenter for Membrane and Cell Physiology, University of Virginia, Charlottesville, Virginia 22908, United States.
Hsien-Wei YehCenter for Membrane and Cell Physiology, University of Virginia, Charlottesville, Virginia 22908, United States.
Ying XiongCenter for Membrane and Cell Physiology, University of Virginia, Charlottesville, Virginia 22908, United States.
Ho Leung NgDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas 66506, United States.
Hui-Wang AiCenter for Membrane and Cell Physiology, University of Virginia, Charlottesville, Virginia 22908, United States.ORCID 0000-0002-5664-3270

Funding

Red and Far-Red Fluorescent Biosensors for Compartmentalized Redox SignalingR01GM129291 · NIGMS · UNIVERSITY OF VIRGINIA · PI AI, HUIWANG · 2018 to 2021
$2.1M
An Integrated Fluorescent Biosensor, Imaging, and Analysis Toolkit for Islet BiologicsR01DK122253 · NIDDK · UNIVERSITY OF VIRGINIA · PI AI, HUIWANG · 2020 to 2023
$1.6M
Fluorescent Redox Indicators to Image Oxidative Stress in Alzheimer's DiseaseRF1AG077773 · NIA · UNIVERSITY OF VIRGINIA · PI AI, HUIWANG · 2022 to 2022
$1.6M
NIA NIH HHS RF1 AG077773NIDDK NIH HHS R01 DK122253NIGMS NIH HHS R01 GM129291
6 · The paper itself

Abstract

Boronic acid-containing fluorescent molecules have been widely used to sense hydrogen peroxide and peroxynitrite, which are important reactive oxygen and nitrogen species in biological systems. However, it has been challenging to gain specificity. Our previous studies developed genetically encoded, green fluorescent peroxynitrite biosensors by genetically incorporating a boronic acid-containing noncanonical amino acid (ncAA),

Indexed as

Biosensing TechniquesPeroxynitrous AcidRed Fluorescent ProteinAnimalsBoron CompoundsBoronic AcidsFluorescent DyesGreen Fluorescent ProteinsHydrogen PeroxideMammalsPhenylalanine4-boronophenylalanineBoron CompoundsBoronic AcidsecpAppleFluorescent DyesGreen Fluorescent ProteinsHydrogen PeroxidePeroxynitrous AcidPhenylalanineRed Fluorescent Protein

Identifiers

PMID37185019
PMCPMC10330634

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.