Evidence map›Paper›PMID 37184932›Full record

ArticleJournal of biomechanical engineering2023

Overload in a Rat In Vivo Model of Synergist Ablation Induces Tendon Multiscale Structural and Functional Degeneration.

Ellen T Bloom, Lily M Lin, Ryan C Locke, Alyssa Giordani, Erin Krassan, John M Peloquin, Karin Grävare Silbernagel, Justin Parreno, Michael H Santare, Megan L Killian and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomechanical engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Successive tendon injury in anFrontiers in bioengineering and biotechnology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ellen T BloomDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.
Lily M LinDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.
Ryan C LockeDepartment of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104; Translational Musculoskeletal Research Center, CMCVAMC, Philadelphia, PA 19104.
Alyssa GiordaniDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.
Erin KrassanDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.
John M PeloquinDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.
Karin Grävare SilbernagelDepartment of Physical Therapy, University of Delaware, Newark, DE 19716.
Justin ParrenoDepartment of Biological Sciences, University of Delaware, Newark, DE 19716.
Michael H SantareDepartment of Mechanical Engineering, University of Delaware, Newark, DE 19716.
Megan L KillianDepartment of Orthopaedic Surgery, University of Michigan, Ann Arbor, MI 48104.
Dawn M ElliottDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716.

Funding

Predictive Modeling & Optimal Control Framework for Model-Based Epidemic Response in DelawareP20GM103446 · NIGMS · UNIVERSITY OF DELAWARE · PI MELINDA K DUNCAN · 2012 to 2026
$67.2M
Updated confocal microscope in the Imaging Core facility to support biomedical researchP20GM103653 · NIGMS · DELAWARE STATE UNIVERSITY · PI HARRINGTON, MELISSA A · 2012 to 2022
$22.7M
Understanding synovial macrophage inflamm-aging within osteoarthritisP20GM139760 · NIGMS · UNIVERSITY OF DELAWARE · PI DAWN M ELLIOTT · 2021 to 2026
$19.1M
Multiscale tendon damage and aberrant cellular responses in an in vivo model of tendinosisR01AR080059 · NIAMS · UNIVERSITY OF DELAWARE · PI DAWN M ELLIOTT · 2022 to 2026
$2.3M
Anisotropic and Nonlinear Structure-Function of Normal and Degenerate TendonR01AR055598 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI ELLIOTT, DAWN M, SOSLOWSKY, LOUIS J · 2008 to 2011
$1.2M
Novel model of tendinosis to investigate multiscale structure and function of tendon after overloadF31AR078005 · NIAMS · UNIVERSITY OF DELAWARE · PI BLOOM, ELLEN · 2021 to 2022
$87k
NIAMS NIH HHS F31 AR078005NIAMS NIH HHS R01 AR080059NIGMS NIH HHS P20 GM103446NIGMS NIH HHS P20 GM103653NIGMS NIH HHS P20 GM139760
6 · The paper itself

Abstract

Tendon degeneration is typically described as an overuse injury with little distinction made between magnitude of load (overload) and number of cycles (overuse). Further, in vivo, animal models of tendon degeneration are mostly overuse models, where tendon damage is caused by a high number of load cycles. As a result, there is a lack of knowledge of how isolated overload leads to degeneration in tendons. A surgical model of synergist ablation (SynAb) overloads the target tendon, plantaris, by ablating its synergist tendon, Achilles. The objective of this study was to evaluate the structural and functional changes that occur following overload of plantaris tendon in a rat SynAb model. Tendon cross-sectional area (CSA) and shape changes were evaluated by longitudinal MR imaging up to 8 weeks postsurgery. Tissue-scale structural changes were evaluated by semiquantified histology and second harmonic generation microscopy. Fibril level changes were evaluated with serial block face scanning electron microscopy (SBF-SEM). Functional changes were evaluated using tension tests at the tissue and microscale using a custom testing system allowing both video and microscopy imaging. At 8 weeks, overloaded plantaris tendons exhibited degenerative changes including increases in CSA, cell density, collagen damage area fraction (DAF), and fibril diameter, and decreases in collagen alignment, modulus, and yield stress. To interpret the differences between overload and overuse in tendon, we introduce a new framework for tendon remodeling and degeneration that differentiates between the inputs of overload and overuse. In summary, isolated overload induces multiscale degenerative structural and functional changes in plantaris tendon.

Indexed as

Achilles TendonMuscle, SkeletalAnimalsCollagenModels, AnimalMuscle Fibers, SkeletalRatsCollagendegenerationhistologymechanical loadingMRIoverloadoverusetendon

Identifiers

PMID37184932
PMCPMC10782872

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.