ArticleThe EMBO journal2023
Acetylation coordinates the crosstalk between carbon metabolism and ammonium assimilation in Salmonella enterica.
Article in The EMBO journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 13 citations in OpenAlex.
- Heat stress-induced degradation of glutamine synthetase rebalances central carbon-nitrogen metabolism and promotes thermotolerance inApplied and environmental microbiology · 2026Article
- Eucalyptus globulus and Cymbopogon flexuosus essential oils antimicrobial and conservative effects against Salmonella enterica serovar typhimurium and possible cytotoxicity: an in vitro and in situ investigation in inoculated pork sirloin.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Proteomic antibacterial characterization of flavonoid xanthohumol and probiotic Clostridium butyricum on pathogenic Clostridioides difficile.Chinese medicine · 2026Article
- Dynamic acetylation of a conserved lysine impacts glycerol kinase activity and abundance in the haloarchaeon Haloferax volcanii.The Journal of biological chemistry · 2026Article
- A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis.NPJ biofilms and microbiomes · 2025Article
- Research on nitrogen transformation pathways of a thermophilic heterotrophic nitrifying bacterial consortium GW7.Frontiers in microbiology · 2025Article
- Characterization and immunoprotective efficacy of a fumarate reductaseFrontiers in microbiology · 2025Article
- Lysine acetylation regulates the AT-rich DNA possession ability of H-NS.Nucleic acids research · 2024Article
- Acetylation coordinates the crosstalk between carbon metabolism and ammonium assimilation in Salmonella enterica.The EMBO journal · 2023Article
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteric bacteria use up to 15% of their cellular energy for ammonium assimilation via glutamine synthetase (GS)/glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) in response to varying ammonium availability. However, the sensory mechanisms for effective and appropriate coordination between carbon metabolism and ammonium assimilation have not been fully elucidated. Here, we report that in Salmonella enterica, carbon metabolism coordinates the activities of GS/GDH via functionally reversible protein lysine acetylation. Glucose promotes Pat acetyltransferase-mediated acetylation and activation of adenylylated GS. Simultaneously, glucose induces GDH acetylation to inactivate the enzyme by impeding its catalytic centre, which is reversed upon GDH deacetylation by deacetylase CobB. Molecular dynamics (MD) simulations indicate that adenylylation is required for acetylation-dependent activation of GS. We show that acetylation and deacetylation occur within minutes of "glucose shock" to promptly adapt to ammonium/carbon variation and finely balance glutamine/glutamate synthesis. Finally, in a mouse infection model, reduced S. enterica growth caused by the expression of adenylylation-mimetic GS is rescued by acetylation-mimicking mutations. Thus, glucose-driven acetylation integrates signals from ammonium assimilation and carbon metabolism to fine-tune bacterial growth control.
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