Evidence map›Paper›PMID 37179509›Full record

ArticleHistochemistry and cell biology2023

Glutamine supplementation accelerates functional recovery of EDL muscles after injury by modulating the expression of S100 calcium-binding proteins.

Audrei R Santos, Tatiana E Koike, Alana M Santana, Natalya C Miranda, Rodrigo A Dell Aquila, Thiago C Silva, Marcelo S Aoki, Elen H Miyabara

Abstract read
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In one paragraph

Article in Histochemistry and cell biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Audrei R SantosDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Tatiana E KoikeDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Alana M SantanaDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Natalya C MirandaDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Rodrigo A Dell AquilaDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Thiago C SilvaDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil.
Marcelo S AokiSchool of Arts, Sciences and Humanities, University of Sao Paulo, Sao Paulo, SP, 03828-000, Brazil.
Elen H MiyabaraDepartment of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof. Lineu Prestes, 2415, Sao Paulo, SP, 05508-000, Brazil. elenm@usp.br.
Universidade de São Paulo · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 314857/2021-4Fundação de Amparo à Pesquisa do Estado de São Paulo 17/09069-4Fundação de Amparo à Pesquisa do Estado de São Paulo 18/24946-4Fundação de Amparo à Pesquisa do Estado de São Paulo 23/00289-2
6 · The paper itself

Abstract

The aim of the current study was to investigate the effect of glutamine supplementation on the expression of HSP70 and the calcium-binding proteins from the S100 superfamily in the recovering extensor digitorum longus (EDL) muscle after injury. Two-month-old Wistar rats were subjected to cryolesion of the EDL muscle and then randomly divided into two groups (with or without glutamine supplementation). Starting immediately after the injury, the supplemented group received daily doses of glutamine (1 g/kg/day, via gavage) for 3 and 10 days orally. Then, muscles were subjected to histological, molecular, and functional analysis. Glutamine supplementation induced an increase in myofiber size of regenerating EDL muscles and prevented the decline in maximum tetanic strength of these muscles evaluated 10 days after injury. An accelerated upregulation of myogenin mRNA levels was detected in glutamine-supplemented injured muscles on day 3 post-cryolesion. The HSP70 expression increased only in the injured group supplemented with glutamine for 3 days. The increase in mRNA levels of NF-κB, the pro-inflammatory cytokines IL-1β and TNF-α, and the calcium-binding proteins S100A8 and S100A9 on day 3 post-cryolesion in EDL muscles was attenuated by glutamine supplementation. In contrast, the decrease in S100A1 mRNA levels in the 3-day-injured EDL muscles was minimized by glutamine supplementation. Overall, our results suggest that glutamine supplementation accelerates the recovery of myofiber size and contractile function after injury by modulating the expression of myogenin, HSP70, NF-κB, pro-inflammatory cytokines, and S100 calcium-binding proteins.

Indexed as

GlutamineNF-kappa BAnimalsCalcium-Binding ProteinsCytokinesDietary SupplementsMuscle ContractionMuscle, SkeletalMyogeninRatsRats, WistarRNA, MessengerCalcium-Binding ProteinsCytokinesGlutamineMyogeninNF-kappa BRNA, MessengerGlutamineHSP70Muscle contractionMuscle regenerationS100 calcium-binding proteins

Identifiers

PMID37179509
OpenAlexW4376598500

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.