Evidence map›Paper›PMID 37178792›Full record

ArticleVirus research2023

Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay.

Jing Miao, Lulu Zuo, Dongmei He, Zhixin Fang, Nicolas Berthet, Chao Yu, Gary Wong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
9.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Strengthening preparedness and response to emerging henipavirus diversity.Frontiers in cellular and infection microbiology · 2026
    Review
  10. Article
  11. CRISPR-Cas12a-Based Isothermal Detection ofInternational journal of molecular sciences · 2025
    Article
  12. Review
  13. Nipah virus: pathogenesis, genome, diagnosis, and treatment.Applied microbiology and biotechnology · 2025
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. CRISPR/Cas system and its application in the diagnosis of animal infectious diseases.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 2 countries.

Jing MiaoViral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shangha 200031, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Lulu ZuoViral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shangha 200031, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Dongmei HeInstitute of Pathogenic Microorganisms, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510440, China.
Zhixin FangBiosafety Laboratory, Guangdong Second Provincial General Hospital, Guangzhou 510317, China.
Nicolas BerthetCentre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Unit of Discovery and Molecular Characterization of Pathogens, Chinese Academy of Sciences, Shanghai 200031, China; Institut Pasteur, Unité Environnement et Risque Infectieux, Cellule d'Intervention Biologique d'Urgence, Université Paris-cite, Paris 75724, France. Electronic address: nicolas.berthet@pasteur.fr.
Chao YuViral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shangha 200031, China. Electronic address: ychao@ips.ac.cn.
Gary WongViral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shangha 200031, China. Electronic address: garyckwong@ips.ac.cn.
Chinese Academy of Sciences · CNInstitut Pasteur of Shanghai · CNGuangdong Provincial Center for Disease Control and Prevention · CNGuangdong Provincial People's Hospital · CNUniversity of Chinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nipah virus (NiV) is a zoonotic pathogen with airborne transmission and high case fatality rates in humans. There is currently no treatment or vaccine against NiV infection approved for humans or animals, therefore early diagnosis is the key to control any potential outbreaks. In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the molecular detection of NiV. The one-pot RPA-CRISPR/Cas13a assay for NiV detection was specific and did not cross-react against other selected (re)-emerging pathogens. The sensitivity of the one-pot RPA-CRISPR/Cas13a assay for NiV detection can detect as little as 10

Indexed as

Nipah VirusRecombinasesAnimalsClustered Regularly Interspaced Short Palindromic RepeatsHumansNucleic Acid Amplification TechniquesNucleotidyltransferasesPolymerase Chain ReactionSensitivity and SpecificityNucleotidyltransferasesRecombinasesCRISPR/Cas13aLateral flow stripsMolecular detectionNipah virusRecombinase polymerase amplification

Identifiers

PMID37178792
PMCPMC10345719
OpenAlexW4376598186

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.