ArticleAnalytical chemistry2023
Real-Time Immunosensor for Small-Molecule Monitoring in Industrial Food Processes.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.Nature communications · 2026Article
- Reversible Sandwich-Based Particle Nanoswitch for Continuous Protein Monitoring at Picomolar Concentrations with Automated Calibration.Angewandte Chemie (International ed. in English) · 2025Article
- Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Understanding Fast and Slow Signal Changes in a Competitive Particle-Based Continuous Biosensor.Analytical chemistry · 2025Article
- Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection.Nature communications · 2025Article
- Nanobody based immunoassay for detection of aquatic virus: Giant salamander iridovirus.Analytica chimica acta · 2025Article
- Continuous Protein Sensing Using Fast-Dissociating Antibody Fragments in Competition-Based Biosensing by Particle Motion.ACS sensors · 2025Article
- How Highly Heterogeneous Sensors with Single-Molecule Resolution can Result in Robust Continuous Monitoring Over Long Time Spans.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Integrating Particle Motion Tracking into Thermal Gel Electrophoresis for Label-Free Sugar Sensing.ACS sensors · 2025Article
- A Review of Advanced Sensor Technologies for Aquatic Products Freshness Assessment in Cold Chain Logistics.Biosensors · 2024Review
- Continuous Biosensor Based on Particle Motion: How Does the Concentration Measurement Precision Depend on Time Scale?ACS sensors · 2024Article
- Molecular Origins of Long-Term Changes in a Competitive Continuous Biosensor with Single-Molecule Resolution.ACS sensors · 2024Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Industrial food processes are monitored to ensure that food is being produced with good quality, yield, and productivity. For developing innovative real-time monitoring and control strategies, real-time sensors are needed that can continuously report chemical and biochemical data of the manufacturing process. Here, we describe a generalizable methodology to develop affinity-based biosensors for the continuous monitoring of small molecules in industrial food processes. Phage-display antibody fragments were developed for the measurement of small molecules, as exemplified with the measurement of glycoalkaloids (GAs) in potato fruit juice. The recombinant antibodies were selected for use in a competition-based biosensor with single-molecule resolution, called biosensing by particle motion, using assay architectures with free particles as well as tethered particles. The resulting sensor measures GAs in the micromolar range, is reversible, has a measurement response time below 5 min, and enables continuous monitoring of GAs in protein-rich solutions for more than 20 h with concentration measurement errors below 15%. The demonstrated biosensor gives the perspective to enable a variety of monitoring and control strategies based on continuous measurement of small molecules in industrial food processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.