ArticleCells2023
Sperm Motility Annotated Genes: Are They Associated with Impaired Fecundity?
Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 10 citations in OpenAlex.
- Genetic alterations in poor-quality individually selected sperm highlight candidate biomarkers for male subfertility.Scientific reports · 2026Article
- Sperm DNA Fragmentation Is Associated with Impaired Directional Motility and Kinematic Efficiency: A CASA-Based Study.Medicina (Kaunas, Lithuania) · 2026Observational
- Small RNA sequencing in individually selected sperm: Biomarkers for male subfertility and predictors of pregnancy success.Non-coding RNA research · 2026Article
- Genetic variants inThe Libyan journal of medicine · 2025Article
- Identifying potential genetic biomarkers for sperm dysfunction through whole-genome sequencing.Scientific reports · 2025Article
- Gene expression alterations in testicular biopsies from males with spermatogenesis arrest identified by transcriptome analysis.PloS one · 2025Article
- Single Sperm RNA signatures reveal MicroRNA biomarkers for male subfertility.Journal of assisted reproduction and genetics · 2024Article
- Determinant genetic markers of semen quality in livestock.Frontiers in endocrinology · 2024Review
- Towards a More Comprehensive Picture of the MicroRNA-23a/b-3p Impact on Impaired Male Fertility.Biology · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm motility is a prerequisite for achieving pregnancy, and alterations in sperm motility, along with sperm count and morphology, are commonly observed in subfertile men. The aim of the study was to determine whether the expression level of genes annotated with the Gene Ontology (GO) term 'sperm motility' differed in sperm collected from healthy men and men diagnosed with oligoasthenozoospermia. Reverse transcription quantitative real-time PCR (RT-qPCR), quantitative mass spectrometry (LC-MS/MS), and enrichment analyses were used to validate a set of 132 genes in 198 men present at an infertility clinic. Out of the 132 studied sperm-motility-associated genes, 114 showed differentially expressed levels in oligoasthenozoospermic men compared to those of normozoospermic controls using an RT-qPCR analysis. Of these, 94 genes showed a significantly lower expression level, and 20 genes showed a significantly higher expression level. An MS analysis of sperm from an independent cohort of healthy and subfertile men identified 692 differentially expressed proteins, of which 512 were significantly lower and 180 were significantly higher in oligoasthenozoospermic men compared to those of the normozoospermic controls. Of the 58 gene products quantified with both techniques, 48 (82.75%) showed concordant regulation. Besides the sperm-motility-associated proteins, the unbiased proteomics approach uncovered several novel proteins whose expression levels were specifically altered in abnormal sperm samples. Among these deregulated proteins, there was a clear overrepresentation of annotation terms related to sperm integrity, the cytoskeleton, and energy-related metabolism, as well as human phenotypes related to spermatogenesis and sperm-related abnormalities. These findings suggest that many of these proteins may serve as diagnostic markers of male infertility. Our study reveals an extended number of sperm-motility-associated genes with altered expression levels in the sperm of men with oligoasthenozoospermia. These genes and/or proteins can be used in the future for better assessments of male factor infertility.
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