Evidence map›Paper›PMID 37174524›Full record

ArticleAnimals : an open access journal from MDPI2023

Extraction and Elevation of Cell-Free DNA under Mastitis and Heat Stress in Dairy Cattle.

Yumei Chen, Zaheer Abbas, Lirong Hu, Ling Kang, Xiao Tan, Qing Xu, Yachun Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yumei ChenInstitute of Life Science and Bioengineering, Beijing Jiaotong University, Haidian, Beijing 100044, China.
Zaheer AbbasInstitute of Life Science and Bioengineering, Beijing Jiaotong University, Haidian, Beijing 100044, China.ORCID 0000-0002-0865-7192
Lirong HuSchool of Animal Science and Technology, China Agricultural University, Haidian, Beijing 100193, China.
Ling KangInstitute of Life Science and Bioengineering, Beijing Jiaotong University, Haidian, Beijing 100044, China.
Xiao TanInstitute of Life Science and Bioengineering, Beijing Jiaotong University, Haidian, Beijing 100044, China.
Qing XuInstitute of Life Science and Bioengineering, Beijing Jiaotong University, Haidian, Beijing 100044, China.ORCID 0000-0002-9680-7356
Yachun WangSchool of Animal Science and Technology, China Agricultural University, Haidian, Beijing 100193, China.ORCID 0000-0003-3629-2802
Beijing Jiaotong University · CNChina Agricultural University · CN

Funding

the China Agriculture Research System of MOF and MARA (Nos. CARS-36) and National Agriculture Key Science & Technology Project (NK20221201). NK20221201
6 · The paper itself

Abstract

In this study, four methods (phenol-chloroform protocol, sodium iodide kit, QIAamp DNA Blood Mini Kit, and TIANamp Micro DNA Kit) were used to extract cell-free DNA (cfDNA) from cattle blood, and the yield and purity of cfDNA varied in four different methods from 0.36 to 0.84 ng/mL for yield and 0.67 to 1.80 (A260/A280) for purity. Compared with other methods, the TIANamp Micro DNA kit performed better in both cfDNA amount and purity (

Indexed as

cell-free DNAextraction methodheat stressHolstein dairy cowsmastitis

Identifiers

PMID37174524
PMCPMC10177014
OpenAlexW4367181636

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.