Evidence map›Paper›PMID 37173548›Full record

ArticleMikrochimica acta2023

Quantification of 25OHD in serum by ID-LC-MS/MS based on oriented immobilization of antibody on magnetic materials.

Keke Yi, Jie Xie, Ziyu Qu, Yanling Lin, Zejian Huang, Tao Peng, Yang Zhao, Rui Zhai, Xiaoyun Gong, You Jiang and 2 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Keke Yi *Technology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Jie Xie *Technology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Ziyu QuTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Yanling LinDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Zejian HuangTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Tao PengTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Yang ZhaoTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Rui ZhaiTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Xiaoyun GongTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
You JiangTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China. jiangyou@nim.ac.cn.
Xinhua DaiTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China. daixh@nim.ac.cn.
Xiang FangTechnology Innovation Center of Mass Spectrometry for State Market Regulation, |Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China. fangxiang@nim.ac.cn.

Funding

National Key Research and Development Program of China 2021YFC2401103National Natural Science Foundation of China 21927812Research Project of the National Institute of Metrology AKY1934Research Project of the National Institute of Metrology AKYZZ2122
6 · The paper itself

Abstract

Magnetic nanomaterials are widely used, but co-adsorption of impurities will lead to saturation. In this study, the aim was to prepare a magnetic nano-immunosorbent material based on orienting immobilization that can purify and separate 25-hydroxyvitamin D (25OHD) from serum and provides a new concept of sample pretreatment technology. Streptococcus protein G (SPG) was modified on the surface of the chitosan magnetic material, and the antibody was oriented immobilized using the ability of SPG to specifically bind to the Fc region of the monoclonal antibody. The antigen-binding domain was fully exposed and made up for the deficiency of the antibody random immobilization. Compared with the antibody in the random binding format, this oriented immobilization strategy can increase the effective activity of the antibody, and the amount of antibody consumed is saved to a quarter of the former. The new method is simple, rapid, and sensitive, without consuming a lot of organic reagents, and can enrich 25OHD after simple protein precipitation. Combining with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the analysis can be completed in less than 30 min. For 25OHD

Indexed as

CalcifediolTandem Mass SpectrometryAntibodies, MonoclonalChromatography, LiquidMagnetic PhenomenaAntibodies, MonoclonalCalcifediol25-Hydroxyvitamin DImmunoaffinity enrichmentLC-MS/MSMagnetic nanoparticlesOriented immobilizationStreptococcus protein G

Identifiers

PMID37173548

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.