ArticlePlanta2023
CRISPR/Cas9-mediated phospholipase C 2 knock-out tomato plants are more resistant to Botrytis cinerea.
Article in Planta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- At the host-pathogen interface: tomato phosphoinositide-specific phospholipase C2 promotes Phytophthora infestans colonization.Plant physiology · 2026Article
- Advances in Genome Editing for Plant Disease Resistance Breeding.Plants (Basel, Switzerland) · 2026Review
- Article
- Winning by Losing: Exploiting Modified Plant Susceptibility Genes to Counteract Necrotrophic Fungal Pathogens.Plant biotechnology journal · 2025Review
- Current status and future trends of eco-friendly management of postharvest fungal decays in tomato fruit.NPJ science of food · 2025Review
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Engineering tomato disease resistance by manipulating susceptibility genes.Frontiers in genome editing · 2025Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Using Transcriptomics to Determine the Mechanism for the Resistance to Fusarium Head Blight of a Wheat-International journal of molecular sciences · 2024Article
- Revolutionizing Tomato Cultivation: CRISPR/Cas9 Mediated Biotic Stress Resistance.Plants (Basel, Switzerland) · 2024Review
- CRISPR-Cas9 Protocol for Efficient Gene Knockout and Transgene-free Plant Generation.Bio-protocol · 2024Article
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionCRISPR/Cas9-mediated Phospholipase C2 knock-out tomato plants are more resistant to Botrytis cinerea than wild-type plants, with less ROS and an increase and reduction of (JA) and (SA)-response marker genes, respectively. Genome-editing technologies allow non-transgenic site-specific mutagenesis of crops, offering a viable alternative to traditional breeding methods. In this study we used CRISPR/Cas9 to inactivate the tomato Phospholipase C2 gene (SlPLC2). Plant PLC activation is one of the earliest responses triggered by different pathogens regulating plant responses that, depending on the plant-pathogen interaction, result in plant resistance or susceptibility. The tomato (Solanum lycopersicum) PLC gene family has six members, named from SlPLC1 to SlPLC6. We previously showed that SlPLC2 transcript levels increased upon xylanase infiltration (fungal elicitor) and that SlPLC2 participates in plant susceptibility to Botrytis cinerea. An efficient strategy to control diseases caused by pathogens is to disable susceptibility genes that facilitate infection. We obtained tomato SlPLC2-knock-out lines with decreased ROS production upon B. cinerea challenge. Since this fungus requires ROS-induced cell death to proliferate, SlPLC2-knock-out plants showed an enhanced resistance with smaller necrotic areas and reduced pathogen proliferation. Thus, we obtained SlPLC2 loss-of-function tomato lines more resistant to B. cinerea by means of CRISPR/Cas9 genome editing technology.
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