Evidence map›Paper›PMID 37171697›Full record

ArticleBiotechnology letters2023

Adipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system.

Vinícius Augusto Simão, Heloisa Brand, Roseli Nunes da Silveira-Antunes, Josianne Thomazini Fukasawa, Jaci Leme, Aldo Tonso, João Tadeu Ribeiro-Paes

Abstract read
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In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
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  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Vinícius Augusto SimãoDepartment of Genetics, School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. viniciusmorma@gmail.com.ORCID http://orcid.org/0000-0002-3915-2795
Heloisa BrandDepartment of Biotechnology, School of Sciences and Letters, São Paulo State University (UNESP), Assis, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-0461-714X
Roseli Nunes da Silveira-AntunesDepartment of Hematology, Hemocenter, School of Medicine of Marilia, Marilia, São Paulo, Brazil.
Josianne Thomazini FukasawaDepartment of Hematology, Hemocenter, School of Medicine of Marilia, Marilia, São Paulo, Brazil.
Jaci LemeCenter for Development and Innovation, Laboratory of Viral Biotechnology, Butantan Institute, São Paulo, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-6103-3290
Aldo TonsoDepartment of Chemical Engineering, Polytechnic School, University of São Paulo, São Paulo, São Paulo, Brazil.ORCID http://orcid.org/0000-0001-8498-7348
João Tadeu Ribeiro-PaesDepartment of Biotechnology, School of Sciences and Letters, São Paulo State University (UNESP), Assis, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-7645-5891

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/03324-4
6 · The paper itself

Abstract

Prior to clinical use, extensive in vitro proliferation of human adipose-derived stem cells (ASCs) is required. Among the current options, spinner-type stirred flasks, which use microcarriers to increase the yield of adherent cells, are recommended. Here, we propose a methodology for ASCs proliferation through cell suspension culture using Cultispher-S

Indexed as

Mesenchymal Stem CellsCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedCulture MediaHumansKineticsCulture MediaAdipose tissueCell proliferationCultispher-S® microcarrierHuman mesenchymal stem stromal cellsKinetic analysisSpinner flaskSuspension culture

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.