Evidence map›Paper›PMID 37170359›Full record

ArticleBMC zoology2022

Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.

Mengke Ni, Zhichao Li, Jing Li, Hui He, Yaling Wang, Yixuan Jiang, Xianwei Wang, Zhuanjian Li, Ming Li, Huifen Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC zoology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Mengke Ni *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Zhichao Li *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Jing LiAnimal Health Supervision Institute of Biyang, Henan, 463700, P. R. China.
Hui HeCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Yaling WangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Yixuan JiangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Xianwei WangHenan Provincial Animal Husbandry General Station, Zhengzhou, 450008, P. R. China.
Zhuanjian LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Ming LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China. liming@henau.edu.cn.
Huifen XuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China. huifen221@126.com.ORCID http://orcid.org/0000-0003-3963-2868
Henan Agricultural University · CNShandong Province Animal Husbandry and Veterinary Bureau · CN

Funding

National Key Research and Development Program of China 2018YFD0502203Special Fund for the Construction of National Modern Agricultural Industrial Park of Biyang County NMAIP-BY-S01Special Fund for the Henan Agriculture Research System HARS-22-13-G1
6 · The paper itself

Abstract

backgroundIn molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression. The purpose of the present study was to screen the most stable reference genes in different muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits. METHODS AND

resultsResults indicated that the most stable reference genes in the muscle tissues of New Zealand white rabbits were HPRT1, ACTB and PPIC, while HPRT1, PPIC, and RPL13A were the most stable reference genes in muscle tissues of Yufeng yellow rabbits. However, in the longissimus dorsi muscle and the abdominal wall muscle of both varieties, the most stable reference genes were HPRT1, RPL13A, and SDHA. In the quadriceps femoris muscle, the most stable reference genes were ACTB, HPRT1, and SDHA. Furthermore, the relative abundance of MYOG, MYH3 and MSTN was used to confirm the suitability and reliability of the selected most stable reference genes and the most unstable reference gene. Results revealed the same expression patterns of these myogenic genes when normalized according to the most stable genes, while normalization against the unstable reference gene altered the observed expression patterns.

conclusionsTaken together, our results demonstrated that the most stable reference genes varied among different muscle tissues and different breeds of rabbits. However, HPRT1, PPIC and SDHA presented high stability among all examined reference genes; thus, the combined analysis of HPRT1/ PPIC/ SDHA gene provides the best reference for RT-qPCR in muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits, while HPRT1 is a better choice than other reference genes when using a single reference gene to assess target gene expression. Our results provide basic data for better measuring target gene expression profiles in muscle tissues of rabbits.

Indexed as

MuscleQuantitative real-time PCRRabbitsReference genes

Identifiers

PMID37170359
PMCPMC10127086
OpenAlexW4311802454

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.