ArticleBMC zoology2022
Mitochondrial gene expression in different organs of Hoplobatrachus rugulosus from China and Thailand under low-temperature stress.
Article in BMC zoology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 15 citations in OpenAlex.
- Environmental RNA reflects seasonal changes in the metabolic activity of stream salamanders.Scientific reports · 2026Article
- Mitochondrial Gene Expression as a Novel Biomarker for Detecting and Discriminating Neurotoxic Pesticide Exposure inInsects · 2026Article
- Intraspecific support for the climate variability hypothesis: oxidative damage in lizards after acute temperature exposure.The Journal of experimental biology · 2025Article
- Differential Mitochondrial Genome Expression of Four Skink Species Under High-Temperature Stress and Selection Pressure Analyses in Scincidae.Animals : an open access journal from MDPI · 2025Article
- Impact of mild hypothermic circulatory arrest on surgical outcomes in acute type a aortic dissection patients: a single-centre study.BMC cardiovascular disorders · 2025Article
- Mitochondrial Protein-Coding Gene Expression in the LizardAnimals : an open access journal from MDPI · 2024Article
- Differential Mitochondrial Genome Expression of Four Hylid Frog Species under Low-Temperature Stress and Its Relationship with Amphibian Temperature Adaptation.International journal of molecular sciences · 2024Article
- Differential Mitochondrial Genome Expression of Three Sympatric Lizards in Response to Low-Temperature Stress.Animals : an open access journal from MDPI · 2024Article
- How Does Mitochondrial Protein-Coding Gene Expression inAnimals : an open access journal from MDPI · 2023Article
- Two-FoldAnimals : an open access journal from MDPI · 2023Article
- Novel mitochondrial genome rearrangements including duplications and extensive heteroplasmy could underlie temperature adaptations in Antarctic notothenioid fishes.Scientific reports · 2023Article
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9 authors at 2 institutions in 2 countries.
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Abstract
backgroundHoplobatrachus rugulosus (Anura: Dicroglossidae) is distributed in China and Thailand and the former can survive substantially lower temperatures than the latter. The mitochondrial genomes of the two subspecies also differ: Chinese tiger frogs (CT frogs) display two identical ND5 genes whereas Thai tiger frogs (TT frogs) have two different ND5 genes. Metabolism of ectotherms is very sensitive to temperature change and different organs have different demands on energy metabolism at low temperatures. Therefore, we conducted studies to understand: (1) the differences in mitochondrial gene expression of tiger frogs from China (CT frogs) versus Thailand (TT frogs); (2) the differences in mitochondrial gene expression of tiger frogs (CT and TT frogs) under short term 24 h hypothermia exposure at 25 °C and 8 °C; (3) the differences in mitochondrial gene expression in three organs (brain, liver and kidney) of CT and TT frogs.
resultsUtilizing RT-qPCR and comparing control groups at 25 °C with low temperature groups at 8 °C, we came to the following results. (1) At the same temperature, mitochondrial gene expression was significantly different in two subspecies. The transcript levels of two identical ND5 of CT frogs were observed to decrease significantly at low temperatures (P < 0.05) whereas the two different copies of ND5 in TT frogs were not. (2) Under low temperature stress, most of the genes in the brain, liver and kidney were down-regulated (except for COI and ATP6 measured in brain and COI measured in liver of CT frogs). (3) For both CT and TT frogs, the changes in overall pattern of mitochondrial gene expression in different organs under low temperature and normal temperature was brain > liver > kidney.
conclusionsWe mainly drew the following conclusions: (1) The differences in the structure and expression of the ND5 gene between CT and TT frogs could result in the different tolerances to low temperature stress. (2) At low temperatures, the transcript levels of most of mitochondrial protein-encoding genes were down-regulated, which could have a significant effect in reducing metabolic rate and supporting long term survival at low temperatures. (3) The expression pattern of mitochondrial genes in different organs was related to mitochondrial activity and mtDNA replication in different organs.
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