ArticleMicrobial cell factories2023
Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
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- Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures.Journal of biological engineering · 2025Article
- Exploration of the Out-of-Phase Phenomenon in Shake Flasks by CFD Calculations of Volumetric Power Input, kBiotechnology and bioengineering · 2025Article
- Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.Bioprocess and biosystems engineering · 2025Article
- A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy.Journal of biological engineering · 2025Article
- Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations.Engineering in life sciences · 2025Review
- Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production.Microbial biotechnology · 2024Article
- Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes.BMC biotechnology · 2024Article
- Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs.Scientific reports · 2024Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundIn research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity. For microbial fermentations, complex substrates and media components are often used. The complex media components can vary in composition, depending on the lot and manufacturing process. These variations can have an immense impact on the results of biological cultivations. The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions. Under these conditions, the organism produces the biopolymer alginate. The focus of the investigation was on the respiration activity, cell growth and alginate production.
resultsYeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated. Significant differences on respiratory activity, growth and production were observed. Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration. On the other hand, the results with well-performing yeast extracts seem to reach a saturation, when their concentration is increased. Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements. Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both. Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
conclusionThe choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations. The proper use of specific yeast extracts allows the cultivation results to be specifically optimised. In addition, supplements can be applied to modify and improve the properties of the alginate. The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level. However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
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