Evidence map›Paper›PMID 37166665›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Fluorescent In Situ Detection of RNA-Protein Interactions in Intact Cells by RNA-PLA.

Tianqi Li, Wei Zhang, Mingyi Xie

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Tianqi LiDepartment of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA.
Wei ZhangDepartment of Genetics, Yale University School of Medicine, New Haven, CT, USA.
Mingyi XieUF Health Cancer Center, University of Florida, Gainesville, FL, USA. mingyi.xie@ufl.edu.
University of Florida · USUniversity of Florida Health · USYale University · US

Funding

VIRAL SMALL RNPS--ROLES IN CELL TRANSFORMATIONP01CA016038 · NCI · YALE UNIVERSITY · PI DIMAIO, DANIEL C. · 1985 to 2020
$25.6M
RNA metabolism mediated by the Integrator complexR35GM128753 · NIGMS · UNIVERSITY OF FLORIDA · PI MINGYI XIE · 2018 to 2026
$4.0M
NCI NIH HHS P01 CA016038NIGMS NIH HHS R35 GM128753
6 · The paper itself

Abstract

RNA-protein proximity ligation assay (RNA-PLA) enables the detection of specific RNA-protein interactions in fixed cells. In RNA-PLA, bridging and ligation of a circular DNA template occurs if the target RNA and protein are within 40 nanometers of each other. The resulting circular template is amplified by rolling circle amplification and abundantly recognized by fluorescent antisense DNA oligonucleotides. This strategy therefore enables localization of RNA-protein interactions in situ with high specificity and sensitivity. Here, we describe the use of RNA-PLA to detect interactions between a nuclear viral RNA and a host RNA-binding protein in Epstein-Barr virus (EBV)-infected B cells.

Indexed as

Epstein-Barr Virus InfectionsRNADNA, CircularHerpesvirus 4, HumanHumansRNA-Binding ProteinsDNA, CircularRNARNA-Binding ProteinsFixed cellIn situ hybridizationProximity ligation assayRibonucleoprotein (RNP)RNA–protein interaction

Identifiers

PMID37166665
PMCPMC11106780
OpenAlexW4376132438

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.