A Simple Method for the Detection of Wybutosine-Modified tRNA

Regina T Nostramo, Anita K Hopper

Open access · greenAbstract read

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Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 87% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Regina T NostramoDepartment of Molecular Genetics, Center for RNA Biology, The Ohio State University, Columbus, OH, USA.

Anita K HopperDepartment of Molecular Genetics, Center for RNA Biology, The Ohio State University, Columbus, OH, USA. hopper.64@osu.edu.

The Ohio State University · US

Funding

tRNA processing and nuclear-cytoplasmic dynamicsR01GM122884 · NIGMS · OHIO STATE UNIVERSITY · PI HOPPER, ANITA K · 2017 to 2024

$2.7M

NIGMS NIH HHS R01 GM122884

6 · The paper itself

Abstract

tRNAs are highly mobile molecules that are trafficked back and forth between the nucleus and cytoplasm by several proteins. However, characterization of the movement of tRNAs and the proteins mediating these movements can be difficult. Here, we describe an easy and cost-effective assay to discover genes that are involved in two specific tRNA trafficking events, retrograde nuclear import and nuclear re-export for yeast, Saccharomyces cerevisiae. This assay, referred to as the hydrochloric acid (HCl)/aniline assay, identifies the presence or absence of a unique modification on tRNA

Indexed as

RNA, Transfer, PheSaccharomyces cerevisiae ProteinsActive Transport, Cell NucleusCell NucleusHydrochloric AcidNucleosidesRNA, TransferSaccharomyces cerevisiaeHydrochloric AcidNucleosidesRNA, TransferRNA, Transfer, PheSaccharomyces cerevisiae ProteinswybutosineAnilineHydrochloric acidNorthern blottRNA re-exporttRNA retrograde nuclear importWybutosineYeast

Identifiers

PMID37166653

DOI10.1007/978-1-0716-3191-1_1

PMCPMC10370157

OpenAlexW4376132862

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.