Evidence map›Paper›PMID 37166635›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Yeast Mitoribosome Purification and Analyses by Sucrose Density Centrifugation and Immunoprecipitation.

Andreas Aufschnaiter, Andreas Carlström, Martin Ott

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Andreas Aufschnaiter *Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Andreas Carlström *Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Martin OttDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. martin.ott@gu.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondrial protein biosynthesis is maintained by an interplay between the mitochondrial ribosome (mitoribosome) and a large set of protein interaction partners. This interactome regulates a diverse set of functions, including mitochondrial gene expression, translation, protein quality control, and respiratory chain assembly. Hence, robust methods to biochemically and structurally analyze this molecular machinery are required to understand the sophisticated regulation of mitochondrial protein biosynthesis. In this chapter, we present detailed protocols for immunoprecipitation, sucrose cushions, and linear sucrose gradients to purify and analyze mitoribosomes and their interaction partners.

Indexed as

Mitochondrial RibosomesSaccharomyces cerevisiaeImmunoprecipitationMitochondriaMitochondrial ProteinsProtein BiosynthesisMitochondrial ProteinsImmunoprecipitationMitochondriaMitoribosomeSucrose cushionSucrose gradientYeast

Identifiers

PMID37166635

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.