Evidence map›Paper›PMID 37163980›Full record

ArticleStem cell reports2023

Single-cell transcriptome analysis of xenotransplanted human retinal organoids defines two migratory cell populations of nonretinal origin.

Ying V Liu, Clayton P Santiago, Akin Sogunro, Gregory J Konar, Ming-Wen Hu, Minda M McNally, Yu-Chen Lu, Miguel Flores-Bellver, Silvia Aparicio-Domingo, Kang V Li and 13 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 2 pooled it
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 32 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Article
  6. A cell fate specification and transition mechanism for human foveolar cone subtype patterning.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  7. Article
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  11. Animal models for the evaluation of retinal stem cell therapies.Progress in retinal and eye research · 2025
    Review
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  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Sequencing: 10X Genomics 3' HT Assay for Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 3 institutions in 1 country.

Ying V LiuWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Clayton P SantiagoSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Akin SogunroDepartment of Biology, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA.
Gregory J KonarWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ming-Wen HuWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Minda M McNallyWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yu-Chen LuWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Miguel Flores-BellverCellSight Ocular Stem Cell and Regeneration Program, Department of Ophthalmology, Sue Anschutz-Rodgers Eye Center, University of Colorado, School of Medicine, Aurora, CO, USA.
Silvia Aparicio-DomingoCellSight Ocular Stem Cell and Regeneration Program, Department of Ophthalmology, Sue Anschutz-Rodgers Eye Center, University of Colorado, School of Medicine, Aurora, CO, USA.
Kang V LiCellSight Ocular Stem Cell and Regeneration Program, Department of Ophthalmology, Sue Anschutz-Rodgers Eye Center, University of Colorado, School of Medicine, Aurora, CO, USA.
Zhuo-Lin LiWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Dzhalal AgakishievWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sarah E HadyniakDepartment of Biology, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA.
Katarzyna A HusseyDepartment of Biology, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA.
Tyler J CreamerInstitute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Linda D OrzolekInstitute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Derek TengWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
M Valeria Canto-SolerCellSight Ocular Stem Cell and Regeneration Program, Department of Ophthalmology, Sue Anschutz-Rodgers Eye Center, University of Colorado, School of Medicine, Aurora, CO, USA.
Jiang QianWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Zheng JiangDepartment of Ophthalmology, Baylor College of Medicine, Houston, TX, USA.
Robert J JohnstonSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biology, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA. Electronic address: robertjohnston@jhu.edu.
Seth BlackshawWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: sethblackshaw@gmail.com.
Mandeep S SinghWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: singhcorrespauth@gmail.com.
Johns Hopkins University · USCellSight Technologies (United States) · USBaylor College of Medicine · US

Funding

Visual Science Training ProgramT32EY007143 · NEI · JOHNS HOPKINS UNIVERSITY · PI Laura Ensign, JEREMY NATHANS · 1995 to 2026
$6.2M
Cone subtype specification in human retinas and organoidsR01EY030872 · NEI · JOHNS HOPKINS UNIVERSITY · PI JOHNSTON, ROBERT JOHN · 2020 to 2023
$2.1M
Developing photoreceptor repair for macular degeneration therapy.R01EY033103 · NEI · JOHNS HOPKINS UNIVERSITY · PI SINGH, MANDEEP · 2021 to 2025
$2.0M
Specification of green and red cone cells in the human eyeF31EY029157 · NEI · JOHNS HOPKINS UNIVERSITY · PI HADYNIAK, SARAH E. · 2019 to 2020
$91k
NEI NIH HHS F31 EY029157NEI NIH HHS R01 EY030872NEI NIH HHS R01 EY033103NEI NIH HHS T32 EY007143
6 · The paper itself

Abstract

Human retinal organoid transplantation could potentially be a treatment for degenerative retinal diseases. How the recipient retina regulates the survival, maturation, and proliferation of transplanted organoid cells is unknown. We transplanted human retinal organoid-derived cells into photoreceptor-deficient mice and conducted histology and single-cell RNA sequencing alongside time-matched cultured retinal organoids. Unexpectedly, we observed human cells that migrated into all recipient retinal layers and traveled long distances. Using an unbiased approach, we identified these cells as astrocytes and brain/spinal cord-like neural precursors that were absent or rare in stage-matched cultured organoids. In contrast, retinal progenitor-derived rods and cones remained in the subretinal space, maturing more rapidly than those in the cultured controls. These data suggest that recipient microenvironment promotes the maturation of transplanted photoreceptors while inducing or facilitating the survival of migratory cell populations that are not normally derived from retinal progenitors. These findings have important implications for potential cell-based treatments of retinal diseases.

Indexed as

Retinal DegenerationSingle-Cell Gene Expression AnalysisAnimalsCell DifferentiationHumansMiceOrganoidsRetinaRetinal Cone Photoreceptor Cellscell invasioncell motilityhereditary retinal diseasesneural progenitorphotoreceptor cellpluripotent stem celltranscriptome

Identifiers

PMID37163980
PMCPMC10202694
OpenAlexW4376133488

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.