ArticlePlant biotechnology journal2023
A dramatic decline in fruit citrate induced by mutagenesis of a NAC transcription factor, AcNAC1.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- The transcriptional regulatory network involved in periderm-specific tanshinones biosynthesis inActa pharmaceutica Sinica. B · 2026Article
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Integrated transcriptomic and physiological analysis reveals cadmium stress responses in kiwifruit rootstockFrontiers in plant science · 2026Article
- Establishment and comparative analysis ofFrontiers in plant science · 2026Article
- Identification of pivotal genes associated with sugar and organic acid metabolism during Prunus Mume fruit ripening via metabolomic and transcriptomic analyses.BMC plant biology · 2025Article
- PbrNAC34a- PbrMYB3/65- PbrACO2 cascade plays a role in citrate difference between the pericarp and cortex tissues of pear (P. bretschneideri Rehd.) fruit.Molecular horticulture · 2025Article
- Metabolomic and Transcriptomic Analyses Provide Insights into Metabolic Networks During Kiyomi Tangors Development and Ripening.Plants (Basel, Switzerland) · 2025Article
- Deterioration in the Quality of 'Xuxiang' Kiwifruit Pulp Caused by Frozen Storage: An Integrated Analysis Based on Phenotype, Color, Antioxidant Activity, and Flavor Compounds.Foods (Basel, Switzerland) · 2025Article
- Aluminum-activated malate transporter family member CsALMT6 mediates fluoride resistance in tea plants (Horticulture research · 2025Article
- CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus.Molecular horticulture · 2025Article
- Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.International journal of molecular sciences · 2025Article
- Identification and evolutionary analysis of NAC transcription factors inFrontiers in plant science · 2025Article
- Divergence in MiRNA targeting of AchAco and its role in citrate accumulation in kiwifruit.BMC plant biology · 2024Article
- The art of tartness: the genetics of organic acid content in fresh fruits.Horticulture research · 2024Article
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10 authors at 4 institutions in 4 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Citrate is a common primary metabolite which often characterizes fruit flavour. The key regulators of citrate accumulation in fruit and vegetables are poorly understood. We systematically analysed the dynamic profiles of organic acid components during the development of kiwifruit (Actinidia spp.). Citrate continuously accumulated so that it became the predominate contributor to total acidity at harvest. Based on a co-expression network analysis using different kiwifruit cultivars, an Al-ACTIVATED MALATE TRANSPORTER gene (AcALMT1) was identified as a candidate responsible for citrate accumulation. Electrophysiological assays using expression of this gene in Xenopus oocytes revealed that AcALMT1 functions as a citrate transporter. Additionally, transient overexpression of AcALMT1 in kiwifruit significantly increased citrate content, while tissues showing higher AcALMT1 expression accumulated more citrate. The expression of AcALMT1 was highly correlated with 17 transcription factor candidates. However, dual-luciferase and EMSA assays indicated that only the NAC transcription factor, AcNAC1, activated AcALMT1 expression via direct binding to its promoter. Targeted CRISPR-Cas9-induced mutagenesis of AcNAC1 in kiwifruit resulted in dramatic declines in citrate levels while malate and quinate levels were not substantially affected. Our findings show that transcriptional regulation of a major citrate transporter, by a NAC transcription factor, is responsible for citrate accumulation in kiwifruit, which has broad implications for other fruits and vegetables.
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