ArticleNature communications2023
Non-enzymatic oligonucleotide ligation in coacervate protocells sustains compartment-content coupling.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.Angewandte Chemie (International ed. in English) · 2026Article
- Sustained formation movement and destruction of coacervates in a waste-free system driven by electricity.Nature communications · 2026Article
- Adaptive Coacervate Microreactors with Broad pH/Thermal Tolerance for Reaction Acceleration.JACS Au · 2026Article
- Article
- Selective membrane wetting of phase-separated giant unilamellar vesicles by coacervate droplets.Nature communications · 2026Article
- Article
- Sequence-Modulated Active Tripeptide Condensates for Tandem Catalysis.Angewandte Chemie (International ed. in English) · 2026Article
- Recent advances in coacervate protocells from passive catalysts to chemically programmable systems.Communications chemistry · 2026Review
- Orchestrating Self-Replication in Artificial Cells with Digital Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Enzymatically reconfigurable liquid crystalline coacervate microdroplets as protocell models.Nature communications · 2025Article
- Article
- Non-equilibrium demixing and dissolution of chiral coacervates via intrinsic catalysis.Nature communications · 2025Article
- Differential stability and dynamics of DNA-based and RNA-based coacervates affect non-enzymatic RNA chemistry.Nature communications · 2025Article
- Dual-Stimulus Programmed Multiphase Separation and Organization in Coacervate Droplets.Angewandte Chemie (International ed. in English) · 2025Article
- High-Yield Prebiotic Polymerization of 2',3'-Cyclic Nucleotides under Wet-Dry Cycling.ACS central science · 2025Article
- Peptide Coacervates Can Protect Sequestered Oligonucleotides from Nucleases and Release Them for Transcription and Translation.Biomacromolecules · 2025Article
- Biomolecule-Based Coacervation: Mechanisms, Applications, and Future Perspectives in Biomedical and Biotechnological Fields.Biomolecules · 2025Review
- Liquid-liquid phase separation-boosted transmembrane delivery in interactive protocell communities.Nature communications · 2025Article
- Light-Responsive Mononucleotide Coacervates.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Adaptive ATP-induced molecular condensation in membranized protocells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern cells are complex chemical compartments tightly regulated by an underlying DNA-encoded program. Achieving a form of coupling between molecular content, chemical reactions, and chassis in synthetic compartments represents a key step to the assembly of evolvable protocells but remains challenging. Here, we design coacervate droplets that promote non-enzymatic oligonucleotide polymerization and that restructure as a result of the reaction dynamics. More specifically, we rationally exploit complexation between end-reactive oligonucleotides able to stack into long physical polymers and a cationic azobenzene photoswitch to produce three different phases-soft solids, liquid crystalline or isotropic coacervates droplets-each of them having a different impact on the reaction efficiency. Dynamical modulation of coacervate assembly and dissolution via trans-cis azobenzene photo-isomerization is used to demonstrate cycles of light-actuated oligonucleotide ligation. Remarkably, changes in the population of polynucleotides during polymerization induce phase transitions due to length-based DNA self-sorting to produce multiphase coacervates. Overall, by combining a tight reaction-structure coupling and environmental responsiveness, our reactive coacervates provide a general route to the non-enzymatic synthesis of polynucleotides and pave the way to the emergence of a primitive compartment-content coupling in membrane-free protocells.
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