ArticleCell reports methods2023
A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy.Journal of biophotonics · 2026Article
- Multispectral extended depth-of-field fluorescence microscopy with co-designed meta-optics and neural reconstruction.Light, science & applications · 2026Article
- An Extended DIY Light-sheet Microscope Enabling Four-color Volumetric Imaging of Whole Biological Samples.Juntendo medical journal · 2026Article
- High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.Biomedical optics express · 2025Article
- PolSpec : Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging.Journal of biophotonics · 2025Article
- Novel opportunities from bioimaging to understand the trafficking and maturation of intracellular pulmonary surfactant and its role in lung diseases.Frontiers in immunology · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyperspectral fluorescence imaging improves multiplexed observations of biological samples by utilizing multiple color channels across the spectral range to compensate for spectral overlap between labels. Typically, spectral resolution comes at a cost of decreased detection efficiency, which both hampers imaging speed and increases photo-toxicity to the samples. Here, we present a high-speed, high-efficiency snapshot spectral acquisition method, based on optical compression of the fluorescence spectra via Fourier transform, that overcomes the challenges of discrete spectral sampling: single-shot hyperspectral phasor camera (SHy-Cam). SHy-Cam captures fluorescence spatial and spectral information in a single exposure with a standard scientific CMOS camera, with photon efficiency of over 80%, easily and with acquisition rates exceeding 30 datasets per second, making it a powerful tool for multi-color
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Registered trials
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