Evidence map›Paper›PMID 37159670›Full record

ArticleCell reports methods2023

Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy.

Suva Roy, Depeng Wang, Andra M Rudzite, Benjamin Perry, Miranda L Scalabrino, Mishek Thapa, Yiyang Gong, Alexander Sher, Greg D Field

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Suva RoyDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
Depeng WangDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Andra M RudziteDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
Benjamin PerryDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Miranda L ScalabrinoDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
Mishek ThapaDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
Yiyang GongDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Alexander SherSanta Cruz Institute for Particle Physics, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Greg D FieldDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.

Funding

VISION RESEARCHP30EY005722 · NEI · DUKE UNIVERSITY · PI Vadim Y Arshavsky · 1985 to 2026
$19.3M
Revealing neural computations through combined optical and electrical recordingsR34NS111645 · NINDS · DUKE UNIVERSITY · PI FIELD, GREGORY DARIN, SHER, ALEXANDER · 2019 to 2019
$680k
Bridging gap in comparative biology using the tree shrew visual systemK99EY032119 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROY, SUVA · 2021 to 2022
$194k
NEI NIH HHS K99 EY032119NEI NIH HHS P30 EY005722NINDS NIH HHS R34 NS111645
6 · The paper itself

Abstract

Visual processing in the retina depends on the collective activity of large ensembles of neurons organized in different layers. Current techniques for measuring activity of layer-specific neural ensembles rely on expensive pulsed infrared lasers to drive 2-photon activation of calcium-dependent fluorescent reporters. We present a 1-photon light-sheet imaging system that can measure the activity in hundreds of neurons in the

Indexed as

MicroscopyNeuronsCalcium, DietaryColoring AgentsLaw EnforcementCalcium, DietaryColoring Agentsbipolar cellscalcium imagingcell typefunctional classificationGCaMPimmunohistochemistrylight-sheet imagingretinaretinal ganglion cellssynapse

Identifiers

PMID37159670
PMCPMC10163030

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.