ArticleNature methods2023
High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Critical point drying of brain tissue for X-ray phase-contrast imaging.Journal of synchrotron radiation · 2026Article
- Synaptic-resolution connectomics: towards large brains and connectomic screening.Nature reviews. Neuroscience · 2026Review
- SmartEM: machine learning-guided electron microscopy.Nature methods · 2026Article
- Nondestructive X-ray tomography of brain tissue ultrastructure.Nature methods · 2025Article
- Optimism for abundant whole-brain connectomes and connectomic screening.Nature methods · 2025Article
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Three-dimensional architecture and linearized mapping of vibrissa follicle afferents.Nature communications · 2025Article
- Array tomography: trails to discovery.Methods in microscopy · 2024Article
- Immersion Fixation and Staining of Multicubic Millimeter Volumes for Electron Microscopy-Based Connectomics of Human Brain Biopsies.Biological psychiatry · 2023Article
- Preserving extracellular space for high-quality optical and ultrastructural studies of whole mammalian brains.Cell reports methods · 2023Article
- Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences.Applied physics letters · 2023Article
- Sample Preparation and Warping Accuracy for Correlative Multimodal Imaging in the Mouse Olfactory Bulb Using 2-Photon, Synchrotron X-Ray and Volume Electron Microscopy.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5-10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
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