ArticleNature communications2023
Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- The AP-1 adaptor complex is required for cell surface modifications and the survival ofInfection and immunity · 2026Article
- Phosphoinositides and inositol phosphates as molecular glues.FEBS letters · 2026Review
- Review
- Article
- Proteome-wide quantification of inositol pyrophosphate-protein interactions.Nature communications · 2026Article
- Mechanistic insights into the regulation of polyphosphate polymerase VTC by the accessory subunit Vtc5.Science China. Life sciences · 2026Article
- The intracellular inositol (pyro)phosphate receptor AtSPX1 reciprocally binds to P1BS DNA.Nature communications · 2026Article
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis.Journal of integrative plant biology · 2025Article
- A linear pathway for inositol pyrophosphate metabolism revealed by 18O labeling and model reduction.PLoS computational biology · 2025Article
- Multilayered Regulation of Fungal Phosphate Metabolism: From Molecular Mechanisms to Ecological Roles in the Global Phosphorus Cycle.Life (Basel, Switzerland) · 2025Review
- Transport and InsPNature communications · 2025Article
- Enzymatic Synthesis of Inositol Pyrophosphates.Methods in molecular biology (Clifton, N.J.) · 2025Article
- (PP)-InsP Affinity Probes for Target Characterization by Immunoblotting and Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.Plant molecular biology · 2024Article
- Cross-kingdom nutrient exchange in the plant-arbuscular mycorrhizal fungus-bacterium continuum.Nature reviews. Microbiology · 2024Review
- Evolution of phosphate scouting in the terrestrial biosphere.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development.PLoS genetics · 2024Article
- Evolution of the SPX gene family and its role in the response mechanism to low phosphorus stress in self-rooted apple stock.BMC genomics · 2024Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many proteins involved in eukaryotic phosphate homeostasis are regulated by SPX domains. In yeast, the vacuolar transporter chaperone (VTC) complex contains two such domains, but mechanistic details of its regulation are not well understood. Here, we show at the atomic level how inositol pyrophosphates interact with SPX domains of subunits Vtc2 and Vtc3 to control the activity of the VTC complex. Vtc2 inhibits the catalytically active VTC subunit Vtc4 by homotypic SPX-SPX interactions via the conserved helix α1 and the previously undescribed helix α7. Binding of inositol pyrophosphates to Vtc2 abrogates this interaction, thus activating the VTC complex. Accordingly, VTC activation is also achieved by site-specific point mutations that disrupt the SPX-SPX interface. Structural data suggest that ligand binding induces reorientation of helix α1 and exposes the modifiable helix α7, which might facilitate its post-translational modification in vivo. The variable composition of these regions within the SPX domain family might contribute to the diversified SPX functions in eukaryotic phosphate homeostasis.
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