Evidence map›Paper›PMID 37155009›Full record

ArticleMolecular biology reports2023

MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma.

Qi-Wei Wang, Ya-Nan Sun, Li-Jun Tan, Jian-Nan Zhao, Xiao-Jie Zhou, Tian-Jiao Yu, Jiang-Tao Liu

RetractedOpen access · hybridAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 3 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Qi-Wei WangDepartment of Otolaryngology, Head and Neck Surgery, Harbin Medical University, Harbin, People's Republic of China.
Ya-Nan SunDepartment of Otolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, No.246, Xuefu Road, Harbin, 150081, People's Republic of China. syn2767@126.com.
Li-Jun TanDepartment of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
Jian-Nan ZhaoDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
Xiao-Jie ZhouDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
Tian-Jiao YuDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
Jiang-Tao LiuDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China. ljt89103@163.com.
First Affiliated Hospital of Harbin Medical University · CNHarbin Medical University · CNSecond Affiliated Hospital of Harbin Medical University · CN

Funding

Health Commission of Heilongjiang Province 2018019National Natural Science Foundation of China 81272965National Natural Science Foundation of China No. 81772874Natural Science Foundation of Heilongjiang Province H2017033the Scientific Research Innovation Fund of the First Affiliated Hospital of Harbin Medical University No.2021M12
6 · The paper itself

Abstract

backgroundMiRNAs can affect the radiosensitization of head and neck squamous cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect on radiation in laryngeal squamous cell cancer (LSCC).

methodsFirst, we systematically analyzed the role of the miR-125 family in HNSCC using the TCGA database and found that miR-125a-5p is associated with radiotherapy. We then performed comprehensive enrichment analysis of miR-125a-5p and predicted target genes. Then, we performed transfection, cell proliferation assays, reverse transcription polymerase chain reaction, apoptosis assays, micronucleus tests, and western blotting on hep-2 cells selected with puromycin.

resultsMiR-125 family members exhibited significantly different expression in HNSCC. They were significantly associated with tumor-node-metastasis staging, clinical stages, and histological grades. Radiation therapy had a statistically effect on miR-125 family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of miR-125a-5p. The proliferation rate of cells transfected with lentivirus vector expressing miR-125a-5p was significantly reduced compared to the other groups. The radiation effect was enhanced in cells transfected with miR-125a-5p. The ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly higher than that of the Ad-control group. Western blotting analysis revealed that miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes.

conclusionsMiR-125 family members could be prognostic biomarkers of HNSCC and improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the effect of radiotherapy on LSCC.

Indexed as

Head and Neck NeoplasmsMicroRNAsCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticHumansRadiation ToleranceSquamous Cell Carcinoma of Head and NeckTumor Suppressor Protein p53MicroRNAsMIRN125 microRNA, humanTumor Suppressor Protein p53Cancer Genome AtlasHead and neck squamous cell carcinoma prognosisLaryngeal squamous cell cancerRadiotherapy

Identifiers

PMID37155009
PMCPMC10209316
OpenAlexW4375863458

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.