Evidence map›Paper›PMID 37154552›Full record

ArticleJournal of visualized experiments : JoVE2023

Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining.

Dillon Strepay, Rafal Olszewski, Ian Taukulis, J Dixon Johns, Shoujun Gu, Michael Hoa

Open access · greenAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Dillon StrepayAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
Rafal OlszewskiAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
Ian TaukulisAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
J Dixon JohnsAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
Shoujun GuAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
Michael HoaAuditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health; michael.hoa@nih.gov.
National Institutes of Health · USNational Institute on Deafness and Other Communication Disorders · US

Funding

Cellular and Molecular Pathways for Hearing Restoration in the Adult Inner EarZIADC000088 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI HOA, MICHAEL · 2015 to 2025
$14.0M
Intramural NIH HHS ZIA DC000088
6 · The paper itself

Abstract

Endocochlear potential, which is generated by the stria vascularis, is essential to maintain an environment conducive to appropriate hair cell mechanotransduction and ultimately hearing. Pathologies of the stria vascularis can result in a decreased hearing. Dissection of the adult stria vascularis allows for focused single-nucleus capture and subsequent single-nucleus sequencing and immunostaining. These techniques are used to study stria vascularis pathophysiology at the single-cell level. Single-nucleus sequencing can be used in the setting of transcriptional analysis of the stria vascularis. Meanwhile, immunostaining continues to be useful in identifying specific populations of cells. Both methods require proper stria vascularis dissection as a prerequisite, which can prove to be technically challenging.

Indexed as

Mechanotransduction, CellularStria VascularisAnimalsCochleaHearingMice

Identifiers

PMID37154552
PMCPMC10443831
OpenAlexW4366605323

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.