ArticleChemical science2023
Engineering fluorescent protein chromophores with an internal reference for high-fidelity ratiometric G4 imaging in living cells.
Article in Chemical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A G-Quadruplex-Activated Near-Infrared Chemiluminescent Probe for In Situ Hepatic Imaging of the Hepatitis C Virus Genome.Angewandte Chemie (International ed. in English) · 2026Article
- Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation.Angewandte Chemie (International ed. in English) · 2026Article
- Revolutionizing Healthcare With Paper-Based Nucleic Acid Testing.Exploration (Beijing, China) · 2026Review
- Illuminating Mitochondrial RNA G-Quadruplexes as Structural Brakes on RNA Granule Assembly and OXPHOS.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Revealing G4 Structures as Drug Targets: Exploring the Potential of Novel Therapeutic Agents.Research (Washington, D.C.) · 2026Review
- Optical Probes for Cellular Imaging of G-quadruplexes: Beyond Fluorescence Intensity Probes.Angewandte Chemie (International ed. in English) · 2025Review
- 1000 fold Ultra-Photosensitized Fluorescent Protein Mimics Toward Photocatalytic Proximity Labeling and Proteomic Profiling Functions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Responsive DNA artificial cells for contact and behavior regulation of mammalian cells.Nature communications · 2025Article
- In vivo targeted-imaging of mitochondrial acidification in an aristolochic acid I-induced nephrotoxicity mouse model by a fluorescent/photoacoustic bimodal probe.Materials today. Bio · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-quadruplexes (G4s) are significant nucleic acid secondary structures formed by guanine-rich sequences. Many single-emission G4 fluorescent probes that are lit up by inhibiting intramolecular rotation have been reported. However, they are non-fluorescent unless structurally rigidified, making them sensitive to other intracellular crowding and confinement environments in the cell, like viscosity. Ratiometric measurements provide built-in self-calibration for signal correction, enabling more sensitive and reliable detection. Herein, we structurally modulate green fluorescent protein (GFP)-like chromophores by integrating the imidazolidinone scaffold of the GFP chromophore and coumarin 6H, obtaining a G4 responsive dual-emission chromophore, called NHCouI. The red emission signal of NHCouI can specifically respond to parallel G4s, while its green emission signal is inert and acts as an internal reference signal. NHCouI-G4 complexes feature high fluorescence quantum yield and excellent anti-photobleaching properties. NHCouI can self-calibrate the signal and avoid viscosity disturbances within the range of major subcellular organelles during G4 imaging in living cells. It is also applied to reflect the difference between apoptosis and ferroptosis
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Registered trials
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