Evidence map›Paper›PMID 37152154›Full record

ArticleFrontiers in plant science2023

iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation.

Veronika Balakhonova, Tereza Dobisova, Zuzana Benedikty, Klara Panzarova, Jaromir Pytela, Radka Koci, Ioannis Spyroglou, Ingrid Kovacova, Dominique Arnaud, Jan Skalak and 2 more

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In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.8field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Veronika BalakhonovaCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Tereza DobisovaCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Zuzana BenediktyPhoton Systems Instruments, Drasov, Czechia.
Klara PanzarovaPhoton Systems Instruments, Drasov, Czechia.
Jaromir PytelaPhoton Systems Instruments, Drasov, Czechia.
Radka KociPhoton Systems Instruments, Drasov, Czechia.
Ioannis SpyroglouCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Ingrid KovacovaCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Dominique ArnaudCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Jan SkalakCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Martin TrtilekPhoton Systems Instruments, Drasov, Czechia.
Jan HejatkoCEITEC - Central European Institute of Technology, Masaryk University, Brno, Czechia.
Central European Institute of Technology – Masaryk University · CZPhoton Systems Instruments (Czechia) · CZCentral European Institute of Technology · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seedling de-etiolation is one of the key stages of the plant life cycle, characterized by a strong rearrangement of the plant development and metabolism. The conversion of dark accumulated protochlorophyllide to chlorophyll in etioplasts of de-etiolating plants is taking place in order of ns to µs after seedlings illumination, leading to detectable increase of chlorophyll levels in order of minutes after de-etiolation initiation. The highly complex chlorophyll biosynthesis integrates number of regulatory events including light and hormonal signaling, thus making de-etiolation an ideal model to study the underlying molecular mechanisms. Here we introduce the iReenCAM, a novel tool designed for non-invasive fluorescence-based quantitation of early stages of chlorophyll biosynthesis during de-etiolation with high spatial and temporal resolution. iReenCAM comprises customized HW configuration and optimized SW packages, allowing synchronized automated measurement and analysis of the acquired fluorescence image data. Using the system and carefully optimized protocol, we show tight correlation between the iReenCAM monitored fluorescence and HPLC measured chlorophyll accumulation during first 4h of seedling de-etiolation in wild type

Indexed as

Arabidopsischlorophyll biosynthesiscytokininsde-etiolationethylenefluorescenceiReenCAM

Identifiers

PMID37152154
PMCPMC10160634
OpenAlexW4366775187

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.