Evidence map›Paper›PMID 37149534›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis.

Mélissa Robb, Joanne K Hobbs, Alisdair B Boraston

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Mélissa RobbDepartment of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
Joanne K HobbsDepartment of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
Alisdair B BorastonDepartment of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada. boraston@uvic.ca.

Funding

CIHR MOP 130305
6 · The paper itself

Abstract

Fluorophore-assisted carbohydrate electrophoresis (FACE) is a method in which a fluorophore is covalently attached to the reducing end of carbohydrates, thereby allowing high-resolution separation by electrophoresis and visualization. This method can be used for carbohydrate profiling and sequencing, as well as for determining the specificity of carbohydrate-active enzymes. Here we describe and demonstrate the use of FACE to separate and visualize the glycans released following digestion of oligosaccharides by glycoside hydrolases (GHs) using two examples: (i) the digestion of chitobiose by the streptococcal β-hexosaminidase GH20C and (ii) the digestion of glycogen by the GH13 member SpuA.

Indexed as

OligosaccharidesPolysaccharidesElectrophoresisElectrophoresis, Polyacrylamide GelFluorescent DyesGlycogenGlycoside HydrolasesFluorescent DyesGlycogenGlycoside HydrolasesOligosaccharidesPolysaccharides8-Aminonaphthalene-1,3,6-trisulphonic acid (ANTS)Carbohydrate-active enzymesElectrophoresisFluorophoreGlycan digestionGlycoside hydrolases

Identifiers

PMID37149534

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.