Evidence map›Paper›PMID 37149528›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Surface Plasmon Resonance Analysis for Quantifying Protein-Carbohydrate Interactions.

Marie Sofie Møller, Darrell W Cockburn, Casper Wilkens

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marie Sofie MøllerDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark.
Darrell W CockburnDepartment of Food Science, The Pennsylvania State University, University Park, PA, USA.
Casper WilkensDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark. cwil@dtu.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During the past two decades, surface plasmon resonance (SPR) analysis has emerged as an important tool for studying protein-carbohydrate interactions, with several commercial instruments available. Binding affinities in the nM to mM range can be determined; however, there are pitfalls that require careful experimental design to avoid. Here we give an overview of each step in the SPR analysis from immobilization to data analysis, providing key points of consideration that will allow practitioners to achieve reliable and reproducible results.

Indexed as

Biosensing TechniquesSurface Plasmon ResonanceCarbohydratesCarbohydratesBiosensorCarbohydrate-active enzymeCarbohydrate-binding moduleEnzymeProtein-carbohydrate interactionsSurface plasmon resonance

Identifiers

PMID37149528

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.