Evidence map›Paper›PMID 37149524›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions.

Darrell W Cockburn, Casper Wilkens, Birte Svensson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. mSphere · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Darrell W CockburnDepartment of Food Science, The Pennsylvania State University, University Park, PA, USA. dwc30@psu.edu.
Casper WilkensDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Birte SvenssonDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Affinity electrophoresis has long been used to study the interaction between proteins and large soluble ligands. The technique has been found to have great utility for the examination of polysaccharide binding by proteins, particularly carbohydrate-binding modules (CBMs). In recent years carbohydrate surface binding sites of proteins, mostly enzymes, have also been investigated by this method. Here we describe a protocol for identifying binding interactions between enzyme catalytic modules and a variety of carbohydrate ligands.

Indexed as

CarbohydratesPolysaccharidesBinding SitesElectrophoresisLigandsProtein BindingCarbohydratesLigandsPolysaccharidesAffinity electrophoresisCarbohydrate-binding moduleDissociation constantPolyacrylamide gel electrophoresisPolysaccharideSurface binding site

Identifiers

PMID37149524

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.