Evidence map›Paper›PMID 37149079›Full record

ArticleActa biomaterialia2023

The cell-assembled extracellular matrix: A focus on the storage stability and terminal sterilization of this human "bio" material.

Diane Potart, Maude Gluais, Alexandra Gaubert, Nicolas Da Silva, Marie Hourques, Marie Sarrazin, Julien Izotte, Léa Mora Charrot, Nicolas L'Heureux

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Diane PotartBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France.
Maude GluaisBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France.
Alexandra GaubertUniversity of Bordeaux, CNRS, UMR 5320, Inserm, UMR121, ANRA, Bordeaux F-33076, France.
Nicolas Da SilvaBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France.
Marie HourquesBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France.
Marie SarrazinBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France.
Julien IzotteAnimal Facility A2, University of Bordeaux, Bordeaux F-33076, France.
Léa Mora CharrotAnimal Facility A2, University of Bordeaux, Bordeaux F-33076, France.
Nicolas L'HeureuxBIOTIS - Laboratory for the Bioengineering of Tissues (UMR Inserm 1026), University of Bordeaux, Inserm, BIOTIS, UMR1026, Campus Carreire, 146 Rue Léo-Saignat, case 45, Bordeaux F-33076, France. Electronic address: Nicolas.lheureux@inserm.fr.

Funding

European Research Council 785908
6 · The paper itself

Abstract

The Cell-Assembled extracellular Matrix (CAM) is an attractive biomaterial because it provided the backbone of vascular grafts that were successfully implanted in patients, and because it can now be assembled in "human textiles". For future clinical development, it is important to consider key manufacturing questions. In this study, the impact of various storage conditions and sterilization methods were evaluated. After 1 year of dry frozen storage, no change in mechanical nor physicochemical properties were detected. However, storage at 4 °C and room temperature resulted in some mechanical changes, especially for dry CAM, but physicochemical changes were minor. Sterilization modified CAM mechanical and physicochemical properties marginally except for hydrated gamma treatment. All sterilized CAM supported cell proliferation. CAM ribbons were implanted subcutaneously in immunodeficient rats to assess the impact of sterilization on the innate immune response. Sterilization accelerated strength loss but no significant difference could be shown at 10 months. Very mild and transient inflammatory responses were observed. Supercritical CO

Indexed as

Extracellular MatrixTissue ScaffoldsAnimalsBiocompatible MaterialsExtracellular Matrix ProteinsHumansRatsSterilizationBiocompatible MaterialsExtracellular Matrix ProteinsCell-assembled extracellular matrixIn vivo remodelingMechanical propertiesPhysicochemical propertiesSterilizationStorage stability

Identifiers

PMID37149079
PMCPMC7614989

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.