ArticleMicroscopy (Oxford, England)2023
A preparation of bacterial outer membrane with osmium tetroxide and uranyl acetate co-stain enables improved structural determination by transmission electron microscopy.
Article in Microscopy (Oxford, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- Outer Membrane Vesicles Derived from Yak Isolates ofAnimals : an open access journal from MDPI · 2026Article
- Bacterial EVs contain small RNAs and transfer RNAs that regulate inflammation in lung infections.Frontiers in immunology · 2026Review
- Human gut commensalGut microbes · 2025Article
- Outer Membrane Vesicles FromJournal of extracellular biology · 2025Article
- Stannous fluoride protects gingival keratinocytes against infection and oxidative stress byFrontiers in dental medicine · 2024Article
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Biological nanoparticles, such as bacterial outer membrane vesicles (OMVs), are routinely characterized through transmission electron microscopy (TEM). In this study, we report a novel method to prepare OMVs for TEM imaging. To preserve vesicular shape and structure, we developed a dual fixation protocol involving osmium tetroxide incubation prior to negative staining with uranyl acetate. Combining osmium tetroxide with uranyl acetate resulted in preservation of sub-50 nm vesicles and improved morphological stability, enhancing characterization of lipid-based nanoparticles by TEM.
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Registered trials
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