ArticleScientific reports2023
Evaluation of methods to assess the quality of cryopreserved Solanaceae pollen.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Morphology-Based Evaluation of Pollen Fertility and Storage Characteristics in MalePlants (Basel, Switzerland) · 2025Article
- Effects of the sucrose concentrations and incubation periods on in vitro pollen germination and pollen tube growth in three rice cultivars.Plant biotechnology (Tokyo, Japan) · 2025Article
- Screening methods for thermotolerance in pollen.Annals of botany · 2025Review
- Effect of ultra-low temperature storage on the viability of pepper pollen and its implications for hybrid breeding.Frontiers in plant science · 2025Article
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding.Plants (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solanaceae pollen cryopreservation is a common practice in the hybrid seed production industry worldwide, enabling effective hybridization across geographical and seasonal limitations. As pollination with low quality pollen can result in significant seed yield loss, monitoring the pollen quality has become an important risk management tool. In this study, pollen quality analysis methods were evaluated for their suitability for routine quality control of cryopreserved pollen batches. The assessments, including pollen viability, pollen germinability and pollen vigor analysis, were conducted in two locations on a diverse set of cryopreserved tomato and pepper pollen batches. While the viability obtained by Impedance Flow Cytometry (IFC) can be interpreted as the pollen's potential to germinate, the in vitro germination assay directly quantifies this functionality under given assay conditions. A linear correlation was found between pollen viability obtained by IFC and in vitro germinability. In conclusion, IFC is the most suitable tool for applications and industries requiring a high degree of automation, throughput, repeatability, and reproducibility. In vitro germination assays are suitable for studies within certain temporal and geographic limitations, due to difficulties in standardization. On the other hand, vigor assessments are not sufficiently addressing the needs of the industry due to poor reproducibility and low throughput.
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