Evidence map›Paper›PMID 37141290›Full record

ArticlePloS one2023

A hybrid technique for measurement of intra/extracellular proteins.

Haiam Edabashi, Radwa Elghadafi, Nischwethaa Rajkanth, Janvi Saurabh Mody, Weiyuan Ma, Serge Dibart, Xiaoren Tang

Abstract read
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Haiam EdabashiDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.ORCID 0000-0001-7582-3261
Radwa ElghadafiDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Nischwethaa RajkanthDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Janvi Saurabh ModyDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Weiyuan MaDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Serge DibartDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Xiaoren TangDepartment of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost.

Indexed as

Sensitivity and SpecificityBlotting, WesternEnzyme-Linked Immunosorbent Assay

Identifiers

PMID37141290
PMCPMC10159171

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.