Evidence map›Paper›PMID 37141247›Full record

ArticlePloS one2023

Identification of the pore-forming and binding domains of the Sneathia vaginalis cytopathogenic toxin A.

Cathyrn K O'Brien, Jacob R Raskin, Ivypel Amankwa Asare, Christine Wei, Joy Ma, Zion T McCoy, Kimberly K Jefferson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Cathyrn K O'BrienDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Jacob R RaskinDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Ivypel Amankwa AsareDepartment of Biomedical Sciences University of Lynchburg, Lynchburg, Virginia, United States of America.
Christine WeiDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Joy MaDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Zion T McCoyDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Kimberly K JeffersonDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.ORCID 0000-0003-1052-1988
Virginia Commonwealth University · USUniversity of Lynchburg · US

Funding

"Role of the cytotoxin, CptA, from the emerging bacterial pathogen Sneathia vaginalis, in pathogenesis"R21AI166168 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI JEFFERSON, KIMBERLY KAY · 2023 to 2024
$427k
NIAID NIH HHS R21 AI166168
6 · The paper itself

Abstract

The association between Sneathia vaginalis and preterm birth is emerging. The Gram-negative anaerobe produces a large exotoxin, the cytopathogenic toxin A (CptA), that forms pores in human epithelial cells and red blood cells. The structure of the toxin has not been determined, but in silico analysis predicts that a large amino-terminal region of the protein is globular and separated from the carboxy-terminal tandem repeats by a disordered region. We found that a recombinant protein consisting of the predicted structured amino-terminal portion of CptA and devoid of the repeat region was sufficient to permeabilize epithelial cells and red blood cells. The repeat region was capable of binding to epithelial cells but did not permeabilize them or lyse red blood cells. CptA is the only S. vaginalis virulence factor that has been examined mechanistically to date, and this analysis sets the foundation for an understanding of how this novel pore-forming toxin exerts its activity.

Indexed as

Premature BirthCell MembraneEpithelial CellsEthylaminesFemaleHumansInfant, NewbornVirulence Factors2-(4-chlorophenylthio)triethylamineEthylaminesVirulence Factors

Identifiers

PMID37141247
PMCPMC10159106
OpenAlexW4368357745

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.