Evidence map›Paper›PMID 37140787›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein.

Gloria I Perez, Michael H Bachmann, Masamitsu Kanada

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Gloria I PerezInstitute for Quantitative Health Science and Engineering (IQ), College of Osteopathic Medicine, Michigan State University, East Lansing, MI, USA.
Michael H BachmannInstitute for Quantitative Health Science and Engineering (IQ), Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
Masamitsu KanadaInstitute for Quantitative Health Science and Engineering (IQ), Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA. kanadama@msu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current methods for characterizing the biodistribution of extracellular vesicles (EVs) are not sensitive enough to track EVs in vivo, despite significant advances over the past decade. Commonly used lipophilic fluorescent dyes are convenient, but lack specificity and yield inaccurate spatiotemporal images in the long-term tracking of EVs. In contrast, protein-based fluorescent or bioluminescent EV reporters have more accurately revealed their distribution in cells and mouse models. Here, we describe a red-shifted bioluminescence resonance energy transfer (BRET) EV reporter, PalmReNL, to analyze the trafficking of small EVs (<200 nm; sEVs) and medium/large EVs (>200 nm; m/lEVs) in mice. Its advantages are that (i) background signals in bioluminescence imaging (BLI) are negligible and (ii) the photons PalmReNL emits have spectral wavelengths longer than 600 nm and can more efficiently penetrate tissues than reporters emitting shorter wavelength light.

Indexed as

Extracellular VesiclesAnimalsDiagnostic ImagingEnergy TransferMiceProteinsTissue DistributionProteinsBiodistributionBioluminescence resonance energy transferDual reporterExosomesExtracellular vesiclesMicrovesiclesm/lEVssEVs

Identifiers

PMID37140787

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.