ArticleAdvanced biology2023
A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells.
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Article in Advanced biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46citing papers in PubMed
7.1field-weighted citation impact, top 2% of its field
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46 citing papers in PubMed, 49 citations in OpenAlex.
- Inflammaging-associated mitochondrial degeneration occurs in hypoglossal motor neurons prior to tongue muscle.GeroScience · 2026Article
- Source-Dependent Mitochondrial Transplantation Drives Schwann Cell Bioenergetic Reprogramming and Peripheral Nerve Regeneration.Advanced healthcare materials · 2026Article
- Junctophilin-2-orchestrated calcium signalosome regulates brown adipocyte thermogenesis and energy metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- 3D EM uncovers mitochondrial network remodeling in residual triple negative breast cancer after conventional chemotherapy treatments.iScience · 2026Article
- ELABELA Targets Mitochondria to Modulate Heart Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Aging Remodels Mitochondrial Architecture Across the Male Reproductive Axis.Research square · 2026Article
- Stress-Encoded Mitochondrial Plasticity: ATF4 Control of Mega-Mitochondria and Nanotunnel Communication.bioRxiv : the preprint server for biology · 2026Article
- The MICOS Complex Regulates Mitochondrial Structure and Oxidative Stress During Age-Dependent Structural Deficits in the Kidney.Aging cell · 2026Article
- A sodium-HIF1α axis coordinates immune metabolic reprogramming and mitochondrial remodeling in salt-sensitive hypertension.Research square · 2026Article
- Structural and mitochondrial dendritic degenerations in old hypoglossal motor neurons.GeroScience · 2026Article
- Microscopy in mitochondrial research - a comprehensive review.Journal of cell science · 2026Review
- The MICOS Complex Regulates Mitochondrial Structure and Oxidative Stress During Age-Dependent Structural Deficits in the Kidney.bioRxiv : the preprint server for biology · 2026Article
- Nutrient State, Aging, and Diet Modulate SAM50-Dependent Mitochondrial Remodeling and Systemic Metabolic Signatures.Research square · 2026Article
- Urolithin A enhances mitochondrial biogenesis-related markers and maximal respiratory capacity during C2C12 differentiation.Frontiers in cell and developmental biology · 2026Article
- The role of MICOS in modulating mitochondrial dynamics and structural changes in vulnerable regions of Alzheimer's Disease.bioRxiv : the preprint server for biology · 2025Article
- Mitochondrial morphological aberrations and redistribution in podocytes with glomerular diseases.Renal failure · 2025Article
- Marf- and Opa1-Dependent Formation of Mitochondrial Network Structure Is Required for Cell Growth and Subsequent Meiosis inInternational journal of molecular sciences · 2025Article
- Deep Learning-Based Automatic Segmentation and Analysis of Mitochondrial Damage by Zika Virus and SARS-CoV-2.Viruses · 2025Article
- Real-Time Monitoring of Mitochondrial pH in HeLa Cells,ACS applied bio materials · 2025Article
- Neuroprotective mitochondria targeted small molecule restores synapses and the distribution of synaptic mitochondria in the hippocampus of APP/PS1 mice.Scientific reports · 2025Article
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Authors and funding
33 authors at 11 institutions in 2 countries.
Antentor HintonDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.ORCID 0000-0002-7730-952X
Prasanna KattiNational Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA.
Trace A ChristensenMicroscopy and Cell Analysis Core Facility, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Margaret MungaiDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Jianqiang ShaoCentral Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA.
Liang ZhangDepartment of Neurology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Sergey TrushinDepartment of Neurology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Ahmad AlghanemDepartment of Internal Medicine, Division of Cardiology, Washington University in St. Louis, 1 Brookings Dr, St. Louis, MO, 63130, USA.
Adam JaspersenMicroscopy and Cell Analysis Core Facility, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Rachel E GerouxDepartment of Neurology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Kit NeikirkCollege of Natural and Health Sciences, University of Hawaii at Hilo, 200 West Kawili St, Hilo, HI, 96720, USA.
Michelle BieteCollege of Natural and Health Sciences, University of Hawaii at Hilo, 200 West Kawili St, Hilo, HI, 96720, USA.
Edgar Garza LopezDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Bryanna ShaoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Dominique StephensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Steven DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, TN, 37208, USA.
Jessica PonceSchool of Medicine, University of Utah, 30 N 1900 E, Salt Lake City, UT, 84132, USA.
Christopher K E BleckNational Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA.
Innes HicsasmazDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Sandra A MurrayDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA, 15206, USA.
Ranthony A C EdmondsDepartment of Mathematics, Ohio State University, 281 W Lane Ave, Columbus, OH, 43210, USA.
Andres DajlesDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Young Do KooDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Serif BacevacDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Jeffrey L SalisburyMicroscopy and Cell Analysis Core Facility, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Renata O PereiraDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
Brian GlancyNational Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA.
Eugenia TrushinaDepartment of Neurology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
E Dale AbelDepartment of Internal Medicine, University of Iowa - Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA, 52242, USA.
University of Iowa · USMayo Clinic · USVanderbilt University · USNational Institutes of Health · USUniversity of Hawaii at Hilo · USFisk University · USKing Saud bin Abdulaziz University for Health Sciences · SAMeharry Medical College · USThe Ohio State University · USUniversity of Pittsburgh · USUniversity of Utah · US
Funding
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI OWEN P MCGUINNESS · 2012 to 2026
$29.3MMULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGYT32DK007563 · NIDDK · VANDERBILT UNIVERSITY · PI Richard M O'Brien · 1988 to 2026
$15.9MElectron Microscopy CoreZICHL005906 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI SYED, ZULFEQHAR · 2009 to 2025
$12.9MMitochondrial Complex I as a Target for Neuroprotection in ADRF1AG055549 · NIA · MAYO CLINIC ROCHESTER · PI TRUSHINA, EUGENIA · 2017 to 2025
$7.0MMedical Scientist Training ProgramT32GM139776 · NIGMS · UNIVERSITY OF IOWA · PI Gordon F Buchanan · 2021 to 2026
$6.4MMetabolic Networks and Pathways Predictive of Sex Differences in AD Risk and Responsiveness to TreatmentRF1AG059093 · NIA · DUKE UNIVERSITY · PI BRINTON, ROBERTA EILEEN, CHANG, RUI · 2018 to 2018
$5.9MPRIDE: Functional and Translational Genomics of Blood DisordersR25HL106365 · NHLBI · AUGUSTA UNIVERSITY · PI PACE, BETTY SUE · 2010 to 2024
$5.2MIntegrated Training in Engineering and DiabetesT32DK101003 · NIDDK · VANDERBILT UNIVERSITY · PI Jamey D. Young · 2014 to 2026
$3.9MAMP-Kinase as a target for neuroprotection in ADRF1AG062135 · NIA · UNIVERSITY OF MINNESOTA · PI LEE, MICHAEL K, TRUSHINA, EUGENIA · 2019 to 2019
$3.2MSmall molecule mitochondria-targeted therapeutics for Huntingtons DiseaseR01NS107265 · NINDS · MAYO CLINIC ROCHESTER · PI TRUSHINA, EUGENIA · 2018 to 2021
$2.2MChronic Alcohol Exposure and Pathophysiology of Alzheimer's Disease.R01AG072898 · NIA · MAYO CLINIC ROCHESTER · PI CHOI, DOO-SUP, TRUSHINA, EUGENIA · 2020 to 2024
$2.0MThe role of the integrated stress response in brown adipose tissue-mediated metabolic adaptationsR01DK125405 · NIDDK · UNIVERSITY OF IOWA · PI PEREIRA ALAMBERT, RENATA · 2020 to 2024
$1.9MNHLBI NIH HHS R01 HL108379NHLBI NIH HHS R25 HL106365NIA NIH HHS R01 AG072898NIA NIH HHS RF1 AG055549NIA NIH HHS RF1 AG059093NIA NIH HHS RF1 AG062135NIDDK NIH HHS P30 DK020593NIDDK NIH HHS R01 DK092065NIDDK NIH HHS R01 DK125405NIDDK NIH HHS T32 DK007563NIDDK NIH HHS T32 DK101003NIEHS NIH HHS R01 ES020715NIGMS NIH HHS T32 GM139776NIH HHS R01DK092065NIH HHS R01HL108379NIH HHS R01NS107265NIH HHS RF1AG55549-06NIH HHS RO1AG062135NIH HHS RO1AG072898NIH HHS RO1AG59093NIH HHS UG3/UH3 NS113776-2NIMHD NIH HHS L60 MD014492NINDS NIH HHS R01 NS107265NINDS NIH HHS UG3 NS113776NINDS NIH HHS UH3 NS113776
Abstract
Mitochondria respond to metabolic demands of the cell and to incremental damage, in part, through dynamic structural changes that include fission (fragmentation), fusion (merging of distinct mitochondria), autophagic degradation (mitophagy), and biogenic interactions with the endoplasmic reticulum (ER). High resolution study of mitochondrial structural and functional relationships requires rapid preservation of specimens to reduce technical artifacts coupled with quantitative assessment of mitochondrial architecture. A practical approach for assessing mitochondrial fine structure using two dimensional and three dimensional high-resolution electron microscopy is presented, and a systematic approach to measure mitochondrial architecture, including volume, length, hyperbranching, cristae morphology, and the number and extent of interaction with the ER is described. These methods are used to assess mitochondrial architecture in cells and tissue with high energy demand, including skeletal muscle cells, mouse brain tissue, and Drosophila muscles. The accuracy of assessment is validated in cells and tissue with deletion of genes involved in mitochondrial dynamics.
Indexed as
MitochondriaMitochondrial MembranesAnimalsCells, CulturedMiceMicroscopy, Electron, Scanningautomated serial block-face SEMfocused ion beam SEMmitochondria-endoplasmic reticulum communicationmitochondrial dynamicsmitochondrial morphologyserial-section TEM
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